Protein Info for IAI47_08275 in Pantoea sp. MT58

Annotation: alpha,alpha-trehalose-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 TIGR02400: alpha,alpha-trehalose-phosphate synthase (UDP-forming)" amino acids 3 to 456 (454 residues), 583.7 bits, see alignment E=1.3e-179 PF00982: Glyco_transf_20" amino acids 18 to 456 (439 residues), 457.6 bits, see alignment E=2.5e-141

Best Hits

Swiss-Prot: 71% identical to OTSA_KLEP7: Trehalose-6-phosphate synthase (otsA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00697, alpha,alpha-trehalose-phosphate synthase (UDP-forming) [EC: 2.4.1.15] (inferred from 98% identity to pva:Pvag_1732)

MetaCyc: 71% identical to trehalose-6-phosphate synthase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalose-phosphate synthase (UDP-forming). [EC: 2.4.1.15]

Predicted SEED Role

"Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)" in subsystem Trehalose Biosynthesis (EC 2.4.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.15

Use Curated BLAST to search for 2.4.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (478 amino acids)

>IAI47_08275 alpha,alpha-trehalose-phosphate synthase (Pantoea sp. MT58)
MSRLVVVSNRVAMPDGSKTSAGGLAVGILDALKSTGGMWFGWNGEISEFTGEEVEDVKVQ
EHEGIEYASFPLSQNDYDLYYCQFSNTVIWPAFHYRLDLVQFQREAWEGYCDVNDSVAQR
LKPLIKPDDIVWIHDYHFLPFAAALRRAGINNRIGFFLHIPFPTPEIFNALPPHKELLEM
LCEYDLLGFQTESDRVAFLDSVSQLTQLQNKGDKKHRAFGNTFMTEVYPIGIEPDSIKEM
AEGPLPPKMAAMKKELGDARNIIACERLDYSKGLPERFLAYEALLENFPEHRGKIRYSQI
APTSRGDVQAYQDIRHQLETEAGRINGKYGTLGWTPLYYLNQHFDRRLLMKIFRLTDVGL
VTPLRDGMNLVAKEYVAAQDPDDPGVLVLSRFAGAANELTSALIVNPYDRDEVAAALDKA
ITMPRTERISRYNDMMAVLRKNDITAWRESFLKDLERIDPRSVDHNTANKVATFPKLA