Protein Info for IAI47_07635 in Pantoea sp. MT58

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01891: amidohydrolase
  • amino acids 4 to 357 (354 residues), 340.5 bits, E=6.3e-106
• PF01546: Peptidase_M20
  • amino acids 63 to 368 (306 residues), 135.7 bits, E=2.1e-43
• PF07687: M20_dimer
  • amino acids 176 to 268 (93 residues), 28.5 bits, E=1.2e-10

Best Hits

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 93% identity to pva:Pvag_1878)

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

• L-lysine biosynthesis II (7/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Protein Sequence (377 amino acids)

>IAI47_07635 amidohydrolase (Pantoea sp. MT58)
MEHLVALRRDLHAHPELGFQEQRTSEMVANFLQQLGMEVHRGIGKTGVVGILKKGSSDRM
IGLRADMDALPMQDQSGTDWQSQAEQVSHACGHDGHTVMLLGAAEKLARDVSFDGSVCFI
FQPAEEGQAGAKAMIEDGLFSRFPCEAVYAIHNWPELPLGQVQTRPGAIMAAADRFDIRV
LGGGGHAAQPHLTCDTLLATSELVVQLNTLVSRALDPCETALLTVTRMQGGFSHNMIPAE
ANITGTVRTFSPTAQDTIEARLRQMAAHITAAHGLQAEVSYRRYYPATINSATEAQFSLE
ALKQAGIPADLAPQPALTSEDFAFMLQARPGAYLWLGSAPCKPLHHAAYDFNDALIPHGI
NVFVALIRQALAEKFPC