Protein Info for IAI47_06400 in Pantoea sp. MT58

Annotation: NADH-quinone oxidoreductase subunit J

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 183 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 28 to 50 (23 residues), see Phobius details amino acids 56 to 76 (21 residues), see Phobius details amino acids 92 to 112 (21 residues), see Phobius details amino acids 133 to 158 (26 residues), see Phobius details PF00499: Oxidored_q3" amino acids 16 to 158 (143 residues), 137 bits, see alignment E=2e-44

Best Hits

Swiss-Prot: 80% identical to NUOJ_ECOLI: NADH-quinone oxidoreductase subunit J (nuoJ) from Escherichia coli (strain K12)

KEGG orthology group: K00339, NADH dehydrogenase I subunit J [EC: 1.6.5.3] (inferred from 100% identity to pva:Pvag_2098)

MetaCyc: 80% identical to NADH:quinone oxidoreductase subunit J (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (183 amino acids)

>IAI47_06400 NADH-quinone oxidoreductase subunit J (Pantoea sp. MT58)
MEFAFYLCGLVAVLTTLRVITHTNPVHALLYLIVSLLSIAGVFFSMGAYFAGALEIIVYA
GAIMVLFVFVVMMLNLGKQTEKQEREWLSPSLWIGPGIVSLLLLVVMIYAILTANDQGID
GTIIDAKAVGISLFGPYVLAVELASMLLLAGLVVAFHIGREERQGEVLSNRNADAAQVKK
ENA