Protein Info for IAI47_06260 in Pantoea sp. MT58

Annotation: bifunctional tetrahydrofolate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 32 to 412 (381 residues), 350 bits, E=1.2e-108
• PF08245: Mur_ligase_M
  • amino acids 54 to 196 (143 residues), 30.5 bits, E=3.4e-11
• PF02875: Mur_ligase_C
  • amino acids 287 to 361 (75 residues), 37.4 bits, E=2.6e-13

Best Hits

Swiss-Prot: 72% identical to FOLC_ECOLI: Dihydrofolate synthase/folylpolyglutamate synthase (folC) from Escherichia coli (strain K12)

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 97% identity to pva:Pvag_2125)

MetaCyc: 72% identical to bifunctional folylpolyglutamate synthetase / dihydrofolate synthetase (Escherichia coli K-12 substr. MG1655)
Tetrahydrofolate synthase. [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; Dihydrofolate synthase. [EC: 6.3.2.17, 6.3.2.12]

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)" in subsystem Folate Biosynthesis (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• superpathway of chorismate metabolism (44/59 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (422 amino acids)

>IAI47_06260 bifunctional tetrahydrofolate synthase/dihydrofolate synthase (Pantoea sp. MT58)
MDNLHLPQATSPLATWLYYLEHLHTQAIELGLDRIRQVALRLDLLKPAPFVFTVAGTNGK
GTTCRTLETLLIAAGYRVGVYSSPHLVRYTERVRVQGEELSEAQHCASFAEIEAGRADIS
LTYFEFGTLSALNLFRQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPD
RESIGREKAGVFRAGKPAVVGEPDMPHSIAVVAQEKGAELLQRNRDWSWQAQAESWSLRD
ALGELNDLPLPQVPLPNAATALVALRVSGLEVSEAQIRASLPLAMLPGRFQTISESPRVI
LDVAHNPHAASYLASQLATLPKRGKVHAVVGMLHDKDIAGTLTALAPMVDAWYCAPLDGP
RGASAEQLLDCLSQGEAFSSVELAWQQARQRAAPQDTVLVCGSFHTVAQVMESIETEKGR
GK