Protein Info for IAI47_05455 in Pantoea sp. MT58

Annotation: NAD-dependent DNA ligase LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 PF01653: DNA_ligase_aden" amino acids 3 to 318 (316 residues), 466.9 bits, see alignment E=1.1e-143 TIGR00575: DNA ligase, NAD-dependent" amino acids 11 to 666 (656 residues), 903.9 bits, see alignment E=2.9e-276 PF03120: DNA_ligase_OB" amino acids 322 to 398 (77 residues), 118.5 bits, see alignment E=3.4e-38 PF03119: DNA_ligase_ZBD" amino acids 407 to 434 (28 residues), 40.8 bits, see alignment (E = 5.6e-14) PF14520: HHH_5" amino acids 447 to 502 (56 residues), 28.8 bits, see alignment 4.9e-10 amino acids 511 to 557 (47 residues), 25.4 bits, see alignment 5.8e-09 PF12826: HHH_2" amino acids 510 to 573 (64 residues), 76.8 bits, see alignment E=4e-25 PF00533: BRCT" amino acids 597 to 668 (72 residues), 61.3 bits, see alignment E=2.9e-20 PF12738: PTCB-BRCT" amino acids 606 to 661 (56 residues), 31 bits, see alignment 7.3e-11

Best Hits

Swiss-Prot: 85% identical to DNLJ_CROS8: DNA ligase (ligA) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 85% identity to ctu:CTU_30080)

MetaCyc: 82% identical to DNA ligase (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.2

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (672 amino acids)

>IAI47_05455 NAD-dependent DNA ligase LigA (Pantoea sp. MT58)
MKSVQDHITELRTTLRHHEYLYHVMDAPEVPDAEYDRLMRELRQLEEEHPDLITPDSPTQ
RVGAAPLTVFEQVRHEVPMLSLDNAFDEATFMAFNKRVQDRLKNSEDITYCCELKLDGAA
VSLMYENGLLVQAATRGDGTTGENITANVRTIRAIPLRLKGDNIPARLEVRGEVFMTQRG
FEKLNEEARRTDGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGFGLLEGGEMPHSHM
ARLQQFKAWGLPVSDRIKRVHNAEEALAFYREIEQQRPNLGFDIDGVVIKVDSQALQEQL
GFVARAPRWAIAFKFPAQEQLTWVRDVEFQVGRTGAITPVARLEPVQVAGVIVSNATLHN
ADEIERLGLRIGDRVVIRRAGDVIPQVVNVVIAERPEETREVVFPAHCPVCGSEVERVEG
ESVTRCTGGLICGAQRKEALKHFVSRRALDVDGMGDKIIDQLVEKEYVSTPADLFRLTAG
KLTGLDRMGPKSAQNVVDALEKAKSTTLARFLYALGIREVGEATAANLANHFGELQKVMD
ADLEALIAVPDVGKVVATHVRNFMEEESNREVIRQLTEEIGIHWPQVVVVKAEEIDSPFA
GKTVVLTGSLSQMNRDDAKAQLVALGAKVSGSVSKKTDLLIAGEAAGSKLAKAQELGIEV
IDEAEMIRLLGA