Protein Info for IAI47_05020 in Pantoea sp. MT58

Annotation: 3-mercaptopyruvate sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 280 transmembrane" amino acids 235 to 256 (22 residues), see Phobius details PF00581: Rhodanese" amino acids 8 to 130 (123 residues), 48.6 bits, see alignment E=4.7e-17 amino acids 165 to 271 (107 residues), 40 bits, see alignment E=2.3e-14

Best Hits

Swiss-Prot: 63% identical to THTM_ECO57: 3-mercaptopyruvate sulfurtransferase (sseA) from Escherichia coli O157:H7

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 96% identity to pva:Pvag_2286)

MetaCyc: 63% identical to 3-mercaptopyruvate sulfurtransferase (Escherichia coli K-12 substr. MG1655)
3-mercaptopyruvate sulfurtransferase. [EC: 2.8.1.2]; Thiosulfate sulfurtransferase. [EC: 2.8.1.2, 2.8.1.1]

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (280 amino acids)

>IAI47_05020 3-mercaptopyruvate sulfurtransferase (Pantoea sp. MT58)
MTTPLFVSADWLQEHYNDERLQVLDARLLPPGMEAVRDIQAEYLAGHLPDAPYFDIEALS
DHTSPYPHMLPRAETFAVAMRELGISSDKHLVVYDEGNLFSAPRAWWMLKTFGVAQVSIL
AGGLQGWKAAGFALASGEVNLPEGEFEAHADESRVKRLTDVLLISHEGGAQIVDARAANR
FNAEVDEPRPGLHRGHIPNSLNVPWNMLVEQGTLKPEAELRRIFADAGVDISQPVVASCG
SGVTAVVLILALTALGARDATLYDGSWGEWGSRDDLPIAK