Protein Info for IAI47_04930 in Pantoea sp. MT58

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1295 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1293 (1291 residues), 2033.1 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 149 (115 residues), 152.4 bits, see alignment E=9.8e-49 PF18072: FGAR-AT_linker" amino acids 170 to 219 (50 residues), 80.1 bits, see alignment (E = 2.7e-26) PF02769: AIRS_C" amino acids 431 to 588 (158 residues), 118.8 bits, see alignment E=5.2e-38 amino acids 832 to 950 (119 residues), 53.7 bits, see alignment E=5.7e-18 PF13507: GATase_5" amino acids 1039 to 1292 (254 residues), 351.7 bits, see alignment E=3.9e-109

Best Hits

Swiss-Prot: 84% identical to PUR4_ECOL6: Phosphoribosylformylglycinamidine synthase (purL) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 71% identity to aap:NT05HA_1480)

MetaCyc: 84% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1295 amino acids)

>IAI47_04930 phosphoribosylformylglycinamidine synthase (Pantoea sp. MT58)
MEILRGSPALSAFRVNKLLTRFQDAHLPVSDIYAEYIHFADVSTPLSADEQSRLQRLLKY
GPSLAEHAPQGRLLLVTPRPGTISPWSSKATDIAHNCGLPQVRRLERGLAFYVQAPQMTE
AQWQTLGTLLHDRMMETVFSDLAQAEQLFAHHTPQPVKSVDLLGEGRQALVQANISLGLA
LADDEIDYLVAAFTSLGRNPNDIELYMFAQANSEHCRHKIFNADWIIDGEKQPKSLFKMI
KNTMEKTPDFVLSAYKDNAAVMEGSEVGRFFADAGESEYRWHQEAAHILMKVETHNHPTA
ISPWPGAATGSGGEIRDEGATGRGSKPKAGLVGFSVSNLRIPGFEQPWEEDFGKPARIVS
ALEIMTDGPLGGAAFNNEFGRPALNGYFRTYEEQVTSHNGTELRGYHKPIMLAGGIGNIR
ADHVQKGEITVGAKLVVLGGPAMNIGLGGGAASSMASGQSDADLDFASVQRDNPEMERRC
QEVIDRCWQLGDENPILFIHDVGAGGLSNAMPELVSDGGRGGRFNLRDILSDEPGMSPLE
VWCNESQERYVMAVAPDQLAQFEAICQRERAPFAVIGEATEELHLSLEDSHFDNKPIDMP
LDVLLGKTPKMTRDVVTQQAQGTELQRDGITLVDAVNRVLHLPAVAEKTFLITIGDRSVT
GMVARDQMVGPWQIPVANCAVTTASLDSYHGEAFALGERSPVALLDFAASGRLAVGEALT
NLAATQIGSLKRVKLSANWMSAAGHPGEDAGLYAAVKAVGEELCPALGITIPVGKDSMSM
KTRWQEGTEQREMTSPLSLVITAFARVEDVRRTVTPQLQADQDNLLLLIDLGNGANTLGA
TALSQVYRQLGDKPADVRDATQLAGFFNAIQALVAQQKLLAYHDRSDGGLLVTLAEMAFA
GHCGIDVDIASLGSDALAALFTEELGAVIQINAADRDAVERILADHGLAASTHLLGSAQP
GDRFVIRSGDSAVYSESRNTLRTWWAETTWQMQRLRDNPACADQEHEAKKNDSDPGLNVN
LTFNPQEDVAAPMIATGARPRVAVLREQGVNSHVEMAAAFDRAGFTAIDVHMSDLLAGRR
GLEAFQALVACGGFSYGDVLGAGEGWAKSILFNPRVRDEFETFFHRPQTLALGVCNGCQM
MSNLRELIPGSDLWPRFVRNQSERFEARFSLVEVAASPSLLLDGMAGSHMPIAVSHGEGF
VEVRDATHLAALESKGLVALRFVDNFGKVTETYPANPNGSPNGITAVTNESGRVTIMMPH
PERVFRTVSNSWHPAEWGEDSPWMRIFRNARRQLG