Protein Info for IAI47_04870 in Pantoea sp. MT58

Annotation: elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 2 to 594 (593 residues), 992.4 bits, E=6.7e-303
• PF00009: GTP_EFTU
  • amino acids 2 to 181 (180 residues), 182.6 bits, E=1.2e-57
• TIGR00231: small GTP-binding protein domain
  • amino acids 6 to 171 (166 residues), 59.5 bits, E=3.4e-20
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 38.9 bits, E=2e-13
• PF00679: EFG_C
  • amino acids 399 to 482 (84 residues), 72.3 bits, E=5.5e-24
• PF06421: LepA_C
  • amino acids 487 to 592 (106 residues), 165.4 bits, E=7.8e-53

Best Hits

Swiss-Prot: 95% identical to LEPA_ERWT9: Elongation factor 4 (lepA) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 95% identity to eam:EAMY_2624)

MetaCyc: 92% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (599 amino acids)

>IAI47_04870 elongation factor 4 (Pantoea sp. MT58)
MKHIRNFSIIAHIDHGKSTLSDRLIQICGGLTAREMAAQVLDSMDLERERGITIKAQSVT
LDYHAKDGQTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EMDLEVVPVLNKIDLPAADPDRAAQEIEDIVGIDATDAVRCSAKTGVGVPEVLERLVRDI
PPPEGDPEGPLQALIIDSWFDNYLGVVSLVRVKNGTMRKGDKIKVLSTGQVYNADRLGIF
TPKRVDRNELNCGEVGWLVCAIKDILGAPVGDTLTTARNPADKALPGFKKVKPQVYAGLF
PISSDDYEAFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
LDLITTAPTVVYEVVTTSGEIVHVDSPSKLPALNNIEELREPIAECHMLLPQEYLGNVIT
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQPSDM
VRVDVLINSERVDALALITHRDNAPYRGRDLVEKMKDLIPRQQFDIAIQAAIGNHIIARS
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDSK