Protein Info for IAI47_04845 in Pantoea sp. MT58

Annotation: L-aspartate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00551: L-aspartate oxidase
  • amino acids 8 to 520 (513 residues), 726.4 bits, E=8e-223
• PF00890: FAD_binding_2
  • amino acids 10 to 392 (383 residues), 346.6 bits, E=6.4e-107
• PF02910: Succ_DH_flav_C
  • amino acids 441 to 521 (81 residues), 54.1 bits, E=4e-18

Best Hits

Swiss-Prot: 80% identical to NADB_ECOLI: L-aspartate oxidase (nadB) from Escherichia coli (strain K12)

KEGG orthology group: K00278, L-aspartate oxidase [EC: 1.4.3.16] (inferred from 96% identity to pva:Pvag_2322)

MetaCyc: 80% identical to L-aspartate oxidase (Escherichia coli K-12 substr. MG1655)
1.5.98.-; L-aspartate oxidase. [EC: 1.4.3.16]

Predicted SEED Role

"L-aspartate oxidase (EC 1.4.3.16)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 1.4.3.16)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• NAD de novo biosynthesis I (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• nicotine biosynthesis (3/9 steps found)
• superpathway of nicotine biosynthesis (4/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Nicotinate and nicotinamide metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.16

Protein Sequence (539 amino acids)

>IAI47_04845 L-aspartate oxidase (Pantoea sp. MT58)
MNVTPDYECDVLIVGSGAAGLSLALRLAKHCQVTVLSKAQVNEGSTLYAQGGIAAVFDET
DSIESHIEDTLIAGAGLCDRDAVSFIASNARHCVQWLIDNGVAFDLDPQAAGDMRYHLTR
EGGHSHRRILHSADATGHAVETTLVSQALSHPNIRILERTNAVDLILSDKIGLPGTRRVV
GAWIWNRNRERVETCRARAVVVATGGAAKVYQYTTNPDIASGDGIAMAWRAGCRVANLEF
NQFHPTCLFHPQAQNFLLTEALRGEGAWLLRPDGSRFMPDFDERAELAPRDIVARAIDHE
MKRLGVDCMYLDISHQPEEFVRAHFPMIYEKLLTFGFDLTKEPIPIVPAAHYTCGGVMVD
QHGQTDVNGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEALIKTLPAIEAVTALP
AWDESQVDDADERVVIQHNWHELRLFMWDYVGIVRTTRRLERALRRINLLQQEIDEYYRH
FRLSNNLLELRNLVQVADLMVRCALERKESRGLHFIRDYPDLLPEALPTILTPPGYMKR