Protein Info for IAI47_04350 in Pantoea sp. MT58

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 transmembrane" amino acids 204 to 219 (16 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 20 to 532 (513 residues), 825.6 bits, see alignment E=7.3e-253 PF04262: Glu_cys_ligase" amino acids 24 to 395 (372 residues), 565.2 bits, see alignment E=2.6e-174

Best Hits

Swiss-Prot: 75% identical to GSH1_YERE8: Glutamate--cysteine ligase (gshA) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 98% identity to pva:Pvag_2394)

MetaCyc: 69% identical to glutamate--cysteine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (535 amino acids)

>IAI47_04350 glutamate--cysteine ligase (Pantoea sp. MT58)
MLNKNKLLSQRGGNVIPDVSKALSWLEAHPNALKGIGRGIERETLRVQENGDLAKSGHPE
SLGSALKHDWITTDFAETLLEFITPVDHSIDHMLAFLRDIHRHVARELGDERMWPFSMPC
VIDDADNIELAQYGTSNIGKMKTLYREGLKNRYGALMQTISGIHYNFSLPLSFWQEWADV
KDQESGKETISAGYLRLIRNYYRFGWVIPYLFGASPAISSSFLQGRESALPFETNDKDTM
WLPYATSLRLSDLGYTNKSQSALGITFNSLEGYVTALKKAIKTPSDEYAAMGAKDADGNW
LQLNTNVLQIENELYAPIRPKRVTRSGEAPSDALLRGGIEYIEVRSLDINPFSAIGVDEN
QVRFLDLFLIWCTLADAPEMSADELQCTRKNWNRVVLEGRKPDQKIAVGCGEAEHSLVEV
GKTLFADLRRVAEVLDSHNQDSTEYQQVCDQLVASFDDPELTYSARILQAMKDNGVTGTG
VALAEQYRHMLCEEPLEVLTEEDFTRQAQESVAAQQQLEANDKLDFEAYLASREG