Protein Info for IAI47_04265 in Pantoea sp. MT58

Annotation: nitrate reductase molybdenum cofactor assembly chaperone

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 TIGR00684: nitrate reductase molybdenum cofactor assembly chaperone" amino acids 8 to 161 (154 residues), 145.6 bits, see alignment E=6e-47 PF02613: Nitrate_red_del" amino acids 41 to 157 (117 residues), 53.9 bits, see alignment E=9.9e-19

Best Hits

Swiss-Prot: 58% identical to NARJ_ECO57: Nitrate reductase molybdenum cofactor assembly chaperone NarJ (narJ) from Escherichia coli O157:H7

KEGG orthology group: K00373, nitrate reductase 1, delta subunit [EC: 1.7.99.4] (inferred from 94% identity to pva:Pvag_2411)

Predicted SEED Role

"Respiratory nitrate reductase delta chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (242 amino acids)

>IAI47_04265 nitrate reductase molybdenum cofactor assembly chaperone (Pantoea sp. MT58)
MMTLRVLSRLLDYPDEALFTHYPDLIAALEEASELNLQQSARLVRFIHELCARPILDVQA
DYCELFDRGRATSLLLFEHVHGESRDRGQAMVDLLEQYRADGLELDSKELPDYLPLYLEY
LACKSDEAARQGLDDIVPILALLAARLEARQSPYADLLRVLLTLSGSAVDPAALKPQVAQ
EARDDTPTALDAVWEEEQIKFLGEQGCASAQQAMHQRRFADAVAPQYLDIAAATARSTGE
SA