Protein Info for IAI47_04260 in Pantoea sp. MT58

Annotation: nitrate reductase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01660: nitrate reductase, beta subunit
  • amino acids 1 to 492 (492 residues), 994.9 bits, E=2.7e-304
• PF13247: Fer4_11
  • amino acids 175 to 271 (97 residues), 126.1 bits, E=1.1e-40
• PF13237: Fer4_10
  • amino acids 178 to 227 (50 residues), 28.2 bits, 3.2e-10
• PF14711: Nitr_red_bet_C
  • amino acids 358 to 436 (79 residues), 100.2 bits, E=8.1e-33

Best Hits

Swiss-Prot: 86% identical to NARH_ECOLI: Respiratory nitrate reductase 1 beta chain (narH) from Escherichia coli (strain K12)

KEGG orthology group: K00371, nitrate reductase 1, beta subunit [EC: 1.7.99.4] (inferred from 97% identity to pva:Pvag_2412)

MetaCyc: 86% identical to nitrate reductase A subunit beta (Escherichia coli K-12 substr. MG1655)
1.97.1.-; RXN0-3501 [EC: 1.7.5.1]; 1.7.5.1 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase beta chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

• NADH to nitrate electron transfer (2/2 steps found)
• nitrate reduction IX (dissimilatory) (2/2 steps found)
• nitrate reduction VIII (dissimilatory) (2/2 steps found)
• nitrate reduction VIIIb (dissimilatory) (2/2 steps found)
• nitrate reduction III (dissimilatory) (1/2 steps found)
• nitrate reduction I (denitrification) (2/5 steps found)
• nitrate reduction VII (denitrification) (2/5 steps found)

KEGG Metabolic Maps

• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Protein Sequence (514 amino acids)

>IAI47_04260 nitrate reductase subunit beta (Pantoea sp. MT58)
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGVGYPHAWEDQQ
KWKGGWIRKINGKLEPRMGSRVGLLSKIFANPDVPALDDYYEPFDFDYQHLHTAPAGKHQ
PIARPRSLITGQRMKKIENGPNWEEILGGEFAKRAQDKNFDNIQKEIYGQFEHTFMMYLP
RLCEHCLNPACVATCPGGAIYKRGEDGIVLIDQDKCRGWRMCLTGCPYKKIYFNWKSGKS
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIEEAAAAETEKDLYQSQLDIFL
DPNDPVVIAQALKDGVAQSVIDAAQRSPVYKMAMEWKLALPLHPEYRTLPMVWYVPPLSP
IQSVVDGGALAHTGVLPDVESLRIPVEYLANLLTAGDTAPVLLALKRMLAMRHYKRAESV
DGEHDIRALEQVGLTEAQAQEMYRYLAIANYEDRFVVPSSHRELAREAFPESKGCGFSFG
DGCHGSDTKFNLFNSRRIDAIDVSKKTSRPEEAS