Protein Info for IAI47_04215 in Pantoea sp. MT58

Annotation: tRNA pseudouridine(13) synthase TruD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00094: tRNA pseudouridine synthase, TruD family
  • amino acids 2 to 337 (336 residues), 256.3 bits, E=3.6e-80
• PF01142: TruD
  • amino acids 5 to 163 (159 residues), 146.2 bits, E=6.6e-47
  • amino acids 183 to 334 (152 residues), 59.4 bits, E=1.5e-20

Best Hits

Swiss-Prot: 68% identical to TRUD_ERWT9: tRNA pseudouridine synthase D (truD) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K06176, tRNA pseudouridine synthase D [EC: 5.4.99.12] (inferred from 77% identity to pam:PANA_3046)

MetaCyc: 63% identical to tRNA pseudouridine13 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11841 [EC: 5.4.99.27]

Predicted SEED Role

"tRNA pseudouridine 13 synthase (EC 4.2.1.-)" in subsystem tRNA processing (EC 4.2.1.-)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Fatty acid biosynthesis
• Limonene and pinene degradation
• Nucleotide sugars metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Porphyrin and chlorophyll metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.-, 5.4.99.12

Use Curated BLAST to search for 4.2.1.- or 5.4.99.12 or 5.4.99.27

Protein Sequence (346 amino acids)

>IAI47_04215 tRNA pseudouridine(13) synthase TruD (Pantoea sp. MT58)
MSLNYLHGTPVASGVIKASPEDFVVVEDLGYGADGEGEHLLVRIRKTGANTRFVAEALAK
YLGIHPRDMSYAGMKDRHAVTEQTLCFRLPGNAMPDLRGFTLEGVEILQAVRHKRKLRIG
ALAGNAFRLVIRQISDRDAVEARLAQIRDAGAPNYFGEQRFGRGGNNLVMAEKWARNEII
VRDRNKRSLMLSAARSALFNQVTSARLQQQGSLTQVLNGDALQLTGRGSWFVAQPEELAD
SQRRVDHHELRITAPLPGRGDAGTQADALAFEQQTLANDTPLLSLIEREKVDAARRAMLV
VPRDMQWRWWDDVTLELTFWLPAGSFATSVVRELLTSADSLDQIDE