Protein Info for IAI47_04050 in Pantoea sp. MT58

Annotation: NADPH-dependent 7-cyano-7-deazaguanine reductase QueF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 TIGR03138: queuine synthase" amino acids 10 to 281 (272 residues), 422.3 bits, see alignment E=5e-131 PF14819: QueF_N" amino acids 21 to 131 (111 residues), 161.7 bits, see alignment E=7.3e-52 PF14489: QueF" amino acids 195 to 267 (73 residues), 88 bits, see alignment E=3.9e-29

Best Hits

Swiss-Prot: 80% identical to QUEF_ERWT9: NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K06879, 7-cyano-7-deazaguanine reductase [EC: 1.7.1.13] (inferred from 96% identity to pva:Pvag_2475)

MetaCyc: 74% identical to 7-cyano-7-deazaguanine reductase (Escherichia coli K-12 substr. MG1655)
PreQ(1) synthase. [EC: 1.7.1.13]

Predicted SEED Role

"NADPH dependent preQ0 reductase (EC 1.7.1.13)" (EC 1.7.1.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (281 amino acids)

>IAI47_04050 NADPH-dependent 7-cyano-7-deazaguanine reductase QueF (Pantoea sp. MT58)
MTTHEQDQALSNLTLGKPTAYHDQYDNSLLQSVPRSLNREPLGLFPDSLPFTGSDIWTLY
ELSWLNSKGLPQVAVGEVVLDAQSRNLIESKSFKLYLNSFNQTRFADWGEVRQTLERDLS
ACAEGEVQVALFRLSEIEGQPVGHFDGCCIDEQDIAIDDYRFNADYLADAAGSEIVEETL
VSHLLKSNCLITNQPDWGSVMIRYTGPRIDREALLRYLVSFRQHNEFHEQCVERIFNDVM
RFCHPEALTVYARYTRRGGLDINPWRTNVPFSPGFSRLVRQ