Protein Info for IAI47_04005 in Pantoea sp. MT58

Annotation: tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 270 transmembrane" amino acids 235 to 255 (21 residues), see Phobius details PF00899: ThiF" amino acids 18 to 260 (243 residues), 142.9 bits, see alignment E=8.6e-46

Best Hits

Swiss-Prot: 81% identical to TCDA_ECOLI: tRNA threonylcarbamoyladenosine dehydratase (tcdA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to pva:Pvag_2484)

MetaCyc: 81% identical to tRNA threonylcarbamoyladenosine dehydratase (Escherichia coli K-12 substr. MG1655)
RXN0-7115

Predicted SEED Role

"CsdL (EC-YgdL) protein of the HesA/MoeB/ThiF family, part of the CsdA-E-L sulfur transfer pathway"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (270 amino acids)

>IAI47_04005 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA (Pantoea sp. MT58)
MKVLNDAWHQRFGGTARLYGQNALQRFADAHFCVIGIGGVGSWAAEALVRTGIGKITLID
MDDVCITNTNRQLHALQGNVGKAKTDVMAERLRAINPDCEVICIDDFITPENTAELMAAG
FDYVIDAIDSVRPKAALIAWCRRNKIPLITTGGAGGQIDPTQIQVADLAKTIQDPLAAKL
RERLKSLGVVKNSKGKLGIDCVFSTEALVYPQADGSVCASRSTAEGPKRMDCTSGFGAAT
MVTATFGFVAVSHALKKFLARAVRQDAVSA