Protein Info for IAI47_03840 in Pantoea sp. MT58

Annotation: lysine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 506 TIGR00499: lysine--tRNA ligase" amino acids 14 to 503 (490 residues), 769.6 bits, see alignment E=6.4e-236 PF01336: tRNA_anti-codon" amino acids 68 to 146 (79 residues), 63 bits, see alignment E=2.1e-21 PF00152: tRNA-synt_2" amino acids 162 to 501 (340 residues), 325.9 bits, see alignment E=2.6e-101

Best Hits

Swiss-Prot: 93% identical to SYK_ERWT9: Lysine--tRNA ligase (lysS) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 100% identity to pva:Pvag_2514)

MetaCyc: 87% identical to lysine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (506 amino acids)

>IAI47_03840 lysine--tRNA ligase (Pantoea sp. MT58)
MSEQQPQSADAAVELNNELKTRREKLSALRANGLAFPNDFRRDSLSNQLLESYGEKSNEE
LEALDIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVSRDDLAEGIYNEQFKKWDLGD
ILGARGKLFRTKTGELSIHCSELRLLTKALRPLPDKFHGLADQETRYRQRYLDLIANEES
RNTFKIRSQIMAGIRQFMVGRDFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAP
ELYLKRLVVGGFDRVFEINRNFRNEGISPRHNPEFTMMELYMAYADYKDLIELTESLFRT
LAQDVLGSTVVPYGEQEFDFGKPFEKLTMREAIKKYRPETDLADLEDFDKAVAIAQSLHI
KVEKSWGLGRVVTEIFEETAEAHLIQPTFITEYPAEVSPLARRNDENPEITDRFEFFIGG
REIGNGFSELNDAEDQAERFLQQVNAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPALRPGSK