Protein Info for IAI47_03100 in Pantoea sp. MT58

Annotation: bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF03710: GlnE" amino acids 35 to 267 (233 residues), 311.6 bits, see alignment E=3.8e-97 amino acids 552 to 803 (252 residues), 341.1 bits, see alignment E=3.9e-106 PF08335: GlnD_UR_UTase" amino acids 296 to 435 (140 residues), 106.8 bits, see alignment E=9.9e-35 amino acids 824 to 912 (89 residues), 29.9 bits, see alignment E=5.3e-11

Best Hits

Swiss-Prot: 72% identical to GLNE_KLEP3: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 78% identity to ebi:EbC_38700)

MetaCyc: 71% identical to fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase (Escherichia coli K-12 substr. MG1655)
GSDEADENYLATION-RXN [EC: 2.7.7.89]; [Glutamate--ammonia-ligase] adenylyltransferase. [EC: 2.7.7.89, 2.7.7.42]

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42 or 2.7.7.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (950 amino acids)

>IAI47_03100 bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase (Pantoea sp. MT58)
MLSALPALLRDQAEAAARQLEIPLDSLSPEQAAGLAFSDFVLENLQQHPDWWQTMLDQPP
QPDEWQHYSAWLNQALNAVDSEASLMRELRLFRRRMLVRVAWMQALQHATTEQSLQQLSV
LAEVLISRARDWVYQDCCRDFGTPCNAEGDAQPLLILGMGKLGGGELNFSSDIDLIFAWP
ENGTTRGGRRELDNAQFFTRMGQRLIKVLDQPTMDGFVYRVDMRLRPFGDSGPLVMSFAA
LEDYYQEQGRDWERYAMVKARLMGDDYDRWSLELQQMLRPFVYRRYIDFSVIQSLRNMKG
MISREVRRRGLKNNIKLGAGGIRETEFIVQVFQLIRGGRERSLQLRSLLPTLEAIKNLAL
LPAEQVDALRDAYLFLRRLENLLQSLGDEQTQTLPEDELHRARLAWAMDCVDWDALMTRL
DDQMAAVRAIFDELIGEDTPDIGDQRELAEFQVLWQDQLEDSELAPLVPQLSDEQRLTLH
QTLEAFRQDINRRTIGPRGRQALDQLMPRLLSEVCPRDDAAVTLSRLTPLLIGVLTRSTY
LELLTEYHGALRHLIRLCAASPMVASQLARHPLLLDELLDPATLYQPTATDAYRDELRQY
LLRIPTEDEEQQLEAVRQFKQAQHLRIAAADIAGTLPVMKVSDHLTWLAEAIIESVVRQA
WNMMVQRYGRPSHLSNEHERGFAVLGYGKLGGWELGYSSDLDLVFLHDCPADAVTEGERS
IDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVSTFEAFEEYQRSEAWT
WEHQALVRARVVFGEATLGERFNVIRQAILSLPRAADALQTEVREMREKMRTHLSNKHKG
RWDIKADAGGITDIEFIAQYLVLRYAAEQPELTRWSDNVRIFELMAKYHKMPADEAQALS
QAYVTLRDALHHRALQELPGHVEPEAYAAERQTVMNSWQRWLVTPASILA