Protein Info for IAI47_02575 in Pantoea sp. MT58

Annotation: N-acetylmuramoyl-L-alanine amidase AmiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF11741: AMIN" amino acids 27 to 115 (89 residues), 31.4 bits, see alignment E=2.6e-11 PF01520: Amidase_3" amino acids 187 to 406 (220 residues), 155.8 bits, see alignment E=1.8e-49 PF01476: LysM" amino acids 451 to 493 (43 residues), 48.9 bits, see alignment 7.4e-17 amino acids 512 to 554 (43 residues), 45.1 bits, see alignment 1.2e-15

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 96% identity to pva:Pvag_2795)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (556 amino acids)

>IAI47_02575 N-acetylmuramoyl-L-alanine amidase AmiB (Pantoea sp. MT58)
MMSRMKIALVTLLLLVCTPLFAATLSDIKVANGDSQATITLNFAGQPAYAFFSLHNPNRV
VLDVNQSGVVQGLPLNFSGENIVKRIRTSTPKDKQSVRLVFELTQAGKTRAVTQRNGNSY
SVVFTITGKAPAITRSAPAPVTAPRPVPTQSADAVNPFNSNPVTSVTSTASTVRPGRAVS
QNDTVIVAIDAGHGGQDPGAIGQRGLKEKNVTIAIARKLRVLLNNDPMFKGVLTRDGDYF
ISVMGRSDVARKANANVLVSIHADAAPSHAATGASVWVLSNRRANNEMANWLEQKEKQSE
LLGGAGDLLANSQADPYLSQAVLDLQFGHSQRVGYDIAQKVLQQLRSVTSLHKRLPEHAS
LGVLRSPDIPSLLVETGFISNSSEERLLGSSAHQDKIAQSIYRGLRTYFLAHPLQSIPKE
ENRPLGSSPAVSVASNPATGVPQYTGVTQRHTVTRGETLSGIAAKYGVSMDTLRGMNALK
RDVVWVGQRLKVPASAVASRTARAAPRGVVRHKVVVGDSLTGIAAHYGVSPKAIMQTNNL
KSTNVMLGQNLKIPAS