Protein Info for IAI47_01535 in Pantoea sp. MT58

Annotation: 16S rRNA (guanine(966)-N(2))-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 199 TIGR00095: 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD" amino acids 11 to 191 (181 residues), 223.6 bits, see alignment E=8.8e-71 PF03602: Cons_hypoth95" amino acids 14 to 190 (177 residues), 213.3 bits, see alignment E=2.3e-67 PF05175: MTS" amino acids 52 to 141 (90 residues), 25.6 bits, see alignment E=8.9e-10

Best Hits

Swiss-Prot: 75% identical to RSMD_ECOLI: Ribosomal RNA small subunit methyltransferase D (rsmD) from Escherichia coli (strain K12)

KEGG orthology group: K08316, ribosomal RNA small subunit methyltransferase D [EC: 2.1.1.171] (inferred from 85% identity to pam:PANA_3726)

MetaCyc: 75% identical to 16S rRNA m2G966 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6515 [EC: 2.1.1.171]

Predicted SEED Role

No annotation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.171

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (199 amino acids)

>IAI47_01535 16S rRNA (guanine(966)-N(2))-methyltransferase (Pantoea sp. MT58)
MNKNPRSTGGAGQIRIIGGQWRGRKLPVPDSAGLRPTTDRVRETLFNWLAPVLPEARCLD
CFAGSGALGLEALSRYAGSATLLELERPVAQQLTKNLQTLRASNGTVIQTNALNWLAQPG
TPFDVVFIDPPFRQGLLEQTLQLLEQNHWLADQALIYVESEVENGAPPVPPEWDLYREKI
AGQVAYRLYQRQQETQDVT