Protein Info for IAI47_01040 in Pantoea sp. MT58
Annotation: threonine ammonia-lyase, biosynthetic
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 86% identical to ILVA_SALTY: L-Threonine dehydratase biosynthetic IlvA (ilvA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 99% identity to pva:Pvag_3107)MetaCyc: 85% identical to threonine deaminase (Escherichia coli K-12 substr. MG1655)
Threonine ammonia-lyase. [EC: 4.3.1.19]
Predicted SEED Role
"Threonine dehydratase biosynthetic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 4.3.1.19)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-threonine metabolism (15/18 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-threonine degradation I (6/6 steps found)
- L-methionine degradation II (3/3 steps found)
- L-threonine degradation V (1/2 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.19
Use Curated BLAST to search for 4.3.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (515 amino acids)
>IAI47_01040 threonine ammonia-lyase, biosynthetic (Pantoea sp. MT58) MAESQPLPDAPGGAEYLRAVLRSPVYEVAQVTPLQVMEKISARLGNTILVKREDRQPVHS FKVRGAYAMIAGLNEEQKARGVVTASAGNHAQGVALSATKLGIKSLIVMPLATADIKVDA VRAFGGEAFLFGANFDEAKAKAIELAEQRGYTFVPPFDHPAVIAGQGTLAMELLQQDAHL DRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVESEDSACLKAALEAGHPVDLPRVGLFAE GVAVKRIGSETFRLCQAYIDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYI QQHEIKGERLAHVLSGANVNFHGLRYVSERCELGEQREALLAVTIPEQQGSFLRFCQTLG GRSITEFNYRYADADKACIFVGVRLTRGVEERSEIISELTSGGYQVVDLSDDEMAKLHVR YMVGGRPSKPLRERLFSFEFPEAPGALLKFLQTLGTHWNISLFHYRSHGTDYGRVLAAFE LGSDEPRFEEHLTDLGYDFHDESQNPAFRFFLSGN