Protein Info for IAI47_00740 in Pantoea sp. MT58

Annotation: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01210: NAD_Gly3P_dh_N
  • amino acids 7 to 162 (156 residues), 179.1 bits, E=1.9e-56
• PF02558: ApbA
  • amino acids 7 to 116 (110 residues), 40.5 bits, E=6.5e-14
• PF07479: NAD_Gly3P_dh_C
  • amino acids 183 to 322 (140 residues), 190.9 bits, E=3.7e-60
• PF20618: GPD_NAD_C_bact
  • amino acids 243 to 309 (67 residues), 87.3 bits, E=2.4e-28

Best Hits

Swiss-Prot: 86% identical to GPDA_ERWT9: Glycerol-3-phosphate dehydrogenase [NAD(P)+] (gpsA) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K00057, glycerol-3-phosphate dehydrogenase (NAD(P)+) [EC: 1.1.1.94] (inferred from 97% identity to pva:Pvag_3171)

MetaCyc: 83% identical to glycerol-3-phosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate dehydrogenase (NAD(P)(+)). [EC: 1.1.1.94]

Predicted SEED Role

"Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 1.1.1.94)

MetaCyc Pathways

• superpathway of phospholipid biosynthesis III (E. coli) (12/12 steps found)
• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (11/13 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• glycerol-3-phosphate shuttle (2/2 steps found)
• 1,3-propanediol biosynthesis (engineered) (6/9 steps found)
• CDP-diacylglycerol biosynthesis III (3/5 steps found)
• glucosylglycerol biosynthesis (3/5 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.94

Protein Sequence (338 amino acids)

>IAI47_00740 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (Pantoea sp. MT58)
METHASISVIGAGSYGTALAITLARNGHPVLLWGHNPTHLAQLQNDRCNTAFLPDVPFPD
NLSLESDLTKAIAASRDLLIVVPSHVFGDVLQQIKPHLRPDSRIVWATKGLEKETGRLLQ
DVAREIVGDTIPLAVISGPTFAKELAAGLPTAIALAATDAQFADDLQQKLHCGKSFRVYN
NPDFIGVQLGGAVKNVIAIGAGISDGIGFGANARTALITRGLTEMSRLGAALGADPTTFM
GMAGLGDLVLTCTDNQSRNRRFGMMLGQGEDVDSAQRIIGQVVEGYRNTKEVKALAARLG
VEMPITEQIYQVLYCEKPARDAALSLLGRTRKDENSTG