Protein Info for IAI47_00460 in Pantoea sp. MT58

Annotation: glutamate--ammonia ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00653: glutamine synthetase, type I
  • amino acids 4 to 468 (465 residues), 714.8 bits, E=2.1e-219
• PF03951: Gln-synt_N
  • amino acids 14 to 95 (82 residues), 113.8 bits, E=2.3e-37
• PF00120: Gln-synt_C
  • amino acids 103 to 466 (364 residues), 473.5 bits, E=4e-146

Best Hits

Swiss-Prot: 90% identical to GLN1B_SHIFL: Glutamine synthetase (glnA) from Shigella flexneri

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 100% identity to pva:Pvag_3223)

MetaCyc: 90% identical to glutamine synthetase (Escherichia coli K-12 substr. MG1655)
Glutamate--ammonia ligase. [EC: 6.3.1.2]

Predicted SEED Role

"Glutamine synthetase type I (EC 6.3.1.2)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Glutamine synthetases or Peptidoglycan Biosynthesis (EC 6.3.1.2)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-aspartate degradation II (aerobic) (3/3 steps found)
• L-aspartate degradation III (anaerobic) (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• ammonia assimilation cycle II (1/2 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.2

Protein Sequence (469 amino acids)

>IAI47_00460 glutamate--ammonia ligase (Pantoea sp. MT58)
MSAEHVLSMMNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNADFFEEGKMFDGSSIGGWKG
INESDMVLMPDATTAVMDPFFEDSTLIIRCDILEPGTMQGYDRDPRSIAKRAEDYLRSSG
IADTVLFGPEPEFFLFDDIRFGSSTSGSHVAIDDIEAAWNTGKEYEGGNKGHRPGLKGGY
FPVPPVDSSQDIRSAMCLTMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYK
YVVHNVAHAYGKTATFMPKPMFGDNGSGMHCHMSLSKGGQNLFAGDKYGGLSEMALFYIG
GIIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVASPKARRIEAR
FPDPAANPYLAFTALLMAGLDGIINKIHPGDAMDKNLYDLPPEEEAEIPKVAGSLEEALN
SLNEDREFLTRGGVFTDESIDAYIELRKAEMDRVRMTPHPVEFELYYSV