Protein Info for IAI47_00245 in Pantoea sp. MT58

Annotation: bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 6 to 68 (63 residues), 29.9 bits, E=9.6e-11
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 6 to 455 (450 residues), 665 bits, E=2.8e-204
• PF12804: NTP_transf_3
  • amino acids 8 to 122 (115 residues), 75.2 bits, E=1.4e-24
• PF00483: NTP_transferase
  • amino acids 8 to 211 (204 residues), 45.4 bits, E=1.5e-15

Best Hits

Swiss-Prot: 83% identical to GLMU_ECOL6: Bifunctional protein GlmU (glmU) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 96% identity to pva:Pvag_3265)

MetaCyc: 83% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• O-antigen building blocks biosynthesis (E. coli) (8/11 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (11/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (456 amino acids)

>IAI47_00245 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU (Pantoea sp. MT58)
MSNSAMSVVILAAGKGTRMYSDLPKVLHTLAGKPMVQHVIDAAKGLGAQHVHLVYGHGGD
RLKATLTDSTLNWVLQAEQLGTGHAMQQAAPAFADDEDIMMLYGDVPLITQATLQRLREA
KPQGGIGLLTVVLDDPTGYGRIVRENDTITGIIEQKDASPAQLTIKEINTGILIANGGDL
KRWLAQLTNNNAQGEYYITDIIALAHQEGRVINAVHPARISETDGVNNRLQLATLERTFQ
AEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDTNVIIEGQVTLGSRVKIGAGCIIKN
SVIGDDCEISPYTVIEDANLATACTVGPFARLRPGSELAQAAHVGNFVEMKKARLGKGSK
AGHLSYLGDAEIGDNVNIGAGTITCNYDGANKFKTVIGDDVFVGSDTQLVAPVNVAPGTT
IAAGTTVMRDVPQAGLVYNRKEQNHKADWQRPVKKK