Protein Info for HSERO_RS21400 in Herbaspirillum seropedicae SmR1

Annotation: tRNA nucleotidyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 PF01743: PolyA_pol" amino acids 4 to 121 (118 residues), 57.4 bits, see alignment E=2.9e-19 PF12627: PolyA_pol_RNAbd" amino acids 147 to 210 (64 residues), 44.5 bits, see alignment E=1.6e-15 PF01966: HD" amino acids 229 to 327 (99 residues), 30.7 bits, see alignment E=5.2e-11

Best Hits

Swiss-Prot: 72% identical to CCA_HERAR: Multifunctional CCA protein (cca) from Herminiimonas arsenicoxydans

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 100% identity to hse:Hsero_4282)

MetaCyc: 54% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IUL9 at UniProt or InterPro

Protein Sequence (410 amino acids)

>HSERO_RS21400 tRNA nucleotidyl transferase (Herbaspirillum seropedicae SmR1)
MKTYTVGGAVRDGLLGLPVKDRDHVVVGATPEQLLALGFRPVGKDFPVFLHPHTQEEYAL
ARTERKTAPGYKGFAFHAAPDVTLEDDLARRDLTINAIAQDEDGTLVDPYHGRADIAARV
FRHVSDAFIEDPVRILRVARFAARFADFTVAPETNALMQRMVEQGEVDALVPERVWQELS
RGLMEQKPSRMFEVLRACGALQRILPELDALWGVPQPEKWHPEIDTGVHVMLVVDWAAAQ
GFTLPIRFAALMHDLGKGTTPPEMWPRHHGHEARSERLAEQVCARLKVPGECRDLALMTA
REHGNVGRAFEMRPDTLVKFFSRCDAFRKPQRFIDMLRAAECDHRGRTGLEQQPFPQAAY
LEGALQAAQQIDAGAIAQAQSQPQRIPEAIQAARTEQVAAFVAQHGAQQP