Protein Info for HSERO_RS20070 in Herbaspirillum seropedicae SmR1

Annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 PF12418: AcylCoA_DH_N" amino acids 4 to 36 (33 residues), 36.6 bits, see alignment (E = 1.1e-12) PF02771: Acyl-CoA_dh_N" amino acids 43 to 159 (117 residues), 38.9 bits, see alignment E=3e-13 PF02770: Acyl-CoA_dh_M" amino acids 164 to 268 (105 residues), 63.6 bits, see alignment E=4.8e-21 PF00441: Acyl-CoA_dh_1" amino acids 278 to 440 (163 residues), 88.1 bits, see alignment E=2.1e-28 PF08028: Acyl-CoA_dh_2" amino acids 295 to 435 (141 residues), 45.4 bits, see alignment E=3e-15 PF12806: Acyl-CoA_dh_C" amino acids 458 to 569 (112 residues), 59.8 bits, see alignment E=8.8e-20

Best Hits

Swiss-Prot: 45% identical to DMDC_RUEPO: 3-methylmercaptopropionyl-CoA dehydrogenase (dmdC) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_4016)

MetaCyc: 45% identical to 3-(methylsulfanyl)propanoyl-CoA dehydrogenase monomer (Ruegeria pomeroyi DSS-3)
RXN-12572 [EC: 1.3.99.41]

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7 or 1.3.99.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISS1 at UniProt or InterPro

Protein Sequence (591 amino acids)

>HSERO_RS20070 acyl-CoA dehydrogenase (Herbaspirillum seropedicae SmR1)
MTMHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNG
IGDREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA
ANHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRT
RAVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDDG
SRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL
GLAEAAYQRASAYARERRQSRAPGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGL
LLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQ
FMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASGLGEQARLLG
EAVDKLRIFASRIAAAAAQAPALPYYVADEMLRLCGHVALGWMWLRAGRRALSAMAQDPL
WYGGKRDAACYHFAFVFAEVQQLSSVIEACLATGLPPLPTDLDHHAAQEAS