Protein Info for HSERO_RS19260 in Herbaspirillum seropedicae SmR1

Annotation: glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 PF02812: ELFV_dehydrog_N" amino acids 48 to 175 (128 residues), 191.4 bits, see alignment E=4.6e-61 PF00208: ELFV_dehydrog" amino acids 194 to 427 (234 residues), 280.1 bits, see alignment E=1.8e-87

Best Hits

Swiss-Prot: 57% identical to DHE3_THEMA: Glutamate dehydrogenase (gdhA) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

KEGG orthology group: K00261, glutamate dehydrogenase (NAD(P)+) [EC: 1.4.1.3] (inferred from 100% identity to hse:Hsero_3858)

MetaCyc: 45% identical to NAD-glutamate dehydrogenase subunit (Peptoniphilus asaccharolyticus)
Glutamate dehydrogenase. [EC: 1.4.1.2]

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" (EC 1.4.1.2, EC 1.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2 or 1.4.1.3 or 1.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IRZ3 at UniProt or InterPro

Protein Sequence (430 amino acids)

>HSERO_RS19260 glutamate dehydrogenase (Herbaspirillum seropedicae SmR1)
MSNVPNHEVPSYLVPHGIGPWGVYLEQIDRVTPYLGSLARWVETLKRPKRMLVVDVPIER
DDGTIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALSAWMTIKNAAVNVPYGGAKG
GIRVDPKTLSRGELQRVTRRYTSEIGIIIGPNKDIPAPDVNTDSQIMAWMMDTYSMNQGS
TSSGVVTGKPISLGGSLGRHEATGRGVFVVGCEAAAKRGLDIKDAKVAVQGFGNVGGIAA
RLFAEAGSKVVAVQDHVTTVFNAGGLDVPALQAYVAKNGSVKGFAGADEITDRAQFWSVD
CDILVPAALEQQITEANANQIKAKIILEGANGPTTPAADDILRDKGVLIVPDVIANAGGV
TVSYFEWVQDFSSFFWTEDEINLRLTRIMREAFAAVWQLADEKKVSLRTAAFIVACTRVL
QAREMRGLYP