Protein Info for HSERO_RS19140 in Herbaspirillum seropedicae SmR1

Annotation: Rieske (2Fe-2S) protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 43 to 124 (82 residues), 45.6 bits, E=5.2e-16
• PF00848: Ring_hydroxyl_A
  • amino acids 165 to 364 (200 residues), 139.6 bits, E=1.4e-44

Best Hits

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_3834)

MetaCyc: 56% identical to hydroxy-succinyl-putrescine hydroxylase (Bordetella bronchiseptica)
RXN-11010

Predicted SEED Role

"Benzoate 1,2-dioxygenase (EC 1.14.12.10)" (EC 1.14.12.10)

MetaCyc Pathways

• benzoate degradation I (aerobic) (2/2 steps found)
• mandelate degradation to acetyl-CoA (13/18 steps found)
• alcaligin biosynthesis (1/5 steps found)
• meta cleavage pathway of aromatic compounds (4/10 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
• toluene degradation IV (aerobic) (via catechol) (5/13 steps found)
• superpathway of aerobic toluene degradation (12/30 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (14/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.10

Use Curated BLAST to search for 1.14.12.10

Sequence Analysis Tools

See D8IRW9 at UniProt or InterPro

Protein Sequence (374 amino acids)

>HSERO_RS19140 Rieske (2Fe-2S) protein (Herbaspirillum seropedicae SmR1)
MSDLATFAKLARSNAQLPVHVYFDEPLYQRELKQLFQQGPRYVGHELMVPEAGDYSTLPW
ENEGRMLVRNAQGIELVSNVCRHRQAKMLDGRGHASNIVCPLHRWTYDLKGELIGAPHFG
QTPCMNLSKTPLQSWNGLLFEANGVDIADKLKNLGVTRDLDFSGYLYDHTEVHECDYNWK
TFIEVYLEDYHVEPFHPGLGNFVSCSDLKWEFGRDYSVQTVGVNHGLIKSGSPTYAKWQE
KLLQFSNGKPPKFGAIWLTLYPNIMVEWYPHVLVVSTLWPTGPQKTTNVVEFYYPEEIAL
FERELVEAERAAYMETCVEDDEIALRMDAGRRILMDRGTSEVGPYQSPMEDGMQHFHEWY
RKQLEVPPGTATTP