Protein Info for HSERO_RS16445 in Herbaspirillum seropedicae SmR1

Annotation: amidophosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 506 TIGR01134: amidophosphoribosyltransferase" amino acids 2 to 466 (465 residues), 519.2 bits, see alignment E=5.9e-160 PF13522: GATase_6" amino acids 64 to 202 (139 residues), 50 bits, see alignment E=4.7e-17 PF13537: GATase_7" amino acids 82 to 214 (133 residues), 45.8 bits, see alignment E=8.6e-16 PF00156: Pribosyltran" amino acids 281 to 395 (115 residues), 38.9 bits, see alignment E=8.1e-14

Best Hits

Swiss-Prot: 59% identical to PUR1_PASMU: Amidophosphoribosyltransferase (purF) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 100% identity to hse:Hsero_3286)

MetaCyc: 58% identical to amidophosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Amidophosphoribosyltransferase. [EC: 2.4.2.14]

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8J1Y4 at UniProt or InterPro

Protein Sequence (506 amino acids)

>HSERO_RS16445 amidophosphoribosyltransferase (Herbaspirillum seropedicae SmR1)
MCGIVGIVSHSPVNQMLYDALLLLQHRGQDAAGIATNHSNGFSMHKANGLVRDVFRTRNM
RSLPGNTGIGQVRYPTAGSSSSEEEAQPFYVNAPFGIILAHNGNLTNAEQLKIEMFKNDR
RHINTDSDTEVLLNVLAHEIQQATTGYSLDPAALFKAVAMVHKRVRGSYAVVAQIAGYGL
LGFRDPYGIRPMCLGFNESDKGVEYMMASESVALEGMGFRFLRDVNPGEAIFIDNDGKLY
NQQCAENPSLNPCAFEYVYLARPDSIIDGASVYATRLKMGEFLADKIRSQFKHGEIDVVM
PIPDSSRPAAMELALKLGIEYREGFIKNRYIGRTFIMPGQALRKKSVRQKLNAIGSEFKG
KNVLLVDDSIVRGTTSREIVQMARESGAKNVIFASAAPPVKFPNVYGIDMPTRDELIAFG
RSEEEVCREITADALVYQDVEALKRAISDANPALKQFEASCFDGHYITGDITQEYLDRIE
YARKNPKPALEDAVRSQLNLNLARVD