Protein Info for HSERO_RS16415 in Herbaspirillum seropedicae SmR1

Annotation: heat shock protein HtpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 transmembrane" amino acids 5 to 26 (22 residues), see Phobius details amino acids 38 to 59 (22 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details amino acids 194 to 218 (25 residues), see Phobius details PF01435: Peptidase_M48" amino acids 80 to 289 (210 residues), 135 bits, see alignment E=1.4e-43

Best Hits

Swiss-Prot: 80% identical to HTPX_JANMA: Protease HtpX homolog (htpX) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K03799, heat shock protein HtpX [EC: 3.4.24.-] (inferred from 100% identity to hse:Hsero_3280)

Predicted SEED Role

"heat shock protease, integral membrane protein"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8J1X8 at UniProt or InterPro

Protein Sequence (291 amino acids)

>HSERO_RS16415 heat shock protein HtpX (Herbaspirillum seropedicae SmR1)
MKRILLFIATNIAVLVVMSVILSLLGVDRFLTRSGLNLPMLLVFSLVVGFTGSIISLLMS
KTMAKWSTGARVIQTPMNGTEAWLLQTVGKLAQRAGIGMPEVAVYDGEPNAFATGAFRDS
ALVAVSTGLLQGMTQDEVEAVLGHEVAHIANGDMVTMTLIQGVVNTFVVFLSRVVGFFVD
SALRRNSDDSGPGIGYTVTVLVCQIVFGIGASLIVAWFSRHREFRADAGAARLLGTPQPM
INALARLGGFSPEGLPQNLAALGISDKPGVMELFSTHPPLEQRIAALRGQR