Protein Info for HSERO_RS16320 in Herbaspirillum seropedicae SmR1

Annotation: FAD-binding molybdopterin dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02963: xanthine dehydrogenase, small subunit
  • amino acids 8 to 475 (468 residues), 690 bits, E=7.1e-212
• PF00111: Fer2
  • amino acids 13 to 74 (62 residues), 27.4 bits, E=5.4e-10
• PF01799: Fer2_2
  • amino acids 87 to 164 (78 residues), 105.3 bits, E=2.8e-34
• PF00941: FAD_binding_5
  • amino acids 202 to 366 (165 residues), 169.4 bits, E=1.3e-53
• PF03450: CO_deh_flav_C
  • amino acids 375 to 474 (100 residues), 121.6 bits, E=3e-39

Best Hits

KEGG orthology group: K13481, xanthine dehydrogenase small subunit [EC: 1.17.1.4] (inferred from 100% identity to hse:Hsero_3262)

Predicted SEED Role

"Xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A (1.17.1.4)" in subsystem Purine Utilization

MetaCyc Pathways

• adenosine nucleotides degradation II (4/5 steps found)
• guanosine nucleotides degradation II (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• ureide biosynthesis (5/7 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• superpathway of purines degradation in plants (9/18 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Sequence Analysis Tools

See D8J1W0 at UniProt or InterPro

Protein Sequence (494 amino acids)

>HSERO_RS16320 FAD-binding molybdopterin dehydrogenase (Herbaspirillum seropedicae SmR1)
MSETHSSIQFYFRGQTHSVAGHSPTRTVLQHLREDLHCTGTKEGCAEGDCGACTVVVGEL
QGEQLALKTVNACIQFLPTLDGRALFTVEDLKQADGSLHPVQQAMVECHGSQCGFCTPGF
VMSLWGLYLRHEQDGVTPSKREIDDALSGNLCRCTGYRPIIDAARRMGELPKVEFDREAI
KAALRSIDQQQPLALTHAGQTFHAPRTLAQLAQLRADLPQARILAGSTDVGLWVTKQMRE
LGDLLYIGRVEELRAMQVRDEMLEIGAGVCLEEAYAEAARHYPEELSELWQRFASLPVRN
AGTLGGNLANGSPIGDSMPWLIALGAQIVLYSVRGQRVMPLEDFYLAYQKSALQADELVQ
AVRIPLPRKELRFRVYKLSKRFDQDISAVCAAFALQMQGDTIVQARIAYGGMAATPKRAA
QAEAVLQGGPWSEAQAQAAMAALAQDYAPLSDMRASAAYRLRTAQNLLYRYWLETRSDAP
LARQELRACAVDAA