Protein Info for HSERO_RS14770 in Herbaspirillum seropedicae SmR1

Annotation: GTP-binding protein HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 2 to 351 (350 residues), 492.3 bits, E=3.5e-152
• PF13167: GTP-bdg_N
  • amino acids 17 to 104 (88 residues), 107.5 bits, E=1.1e-34
• PF16360: GTP-bdg_M
  • amino acids 106 to 184 (79 residues), 95.6 bits, E=5.2e-31
• PF02421: FeoB_N
  • amino acids 191 to 315 (125 residues), 29.3 bits, E=1.4e-10
• PF01926: MMR_HSR1
  • amino acids 192 to 310 (119 residues), 73.7 bits, E=3.4e-24

Best Hits

Swiss-Prot: 56% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 100% identity to hse:Hsero_2947)

MetaCyc: 56% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See D8IZU5 at UniProt or InterPro

Protein Sequence (374 amino acids)

>HSERO_RS14770 GTP-binding protein HflX (Herbaspirillum seropedicae SmR1)
MRAALVGLDFGKNDFAASLDELYLLAKSAGAEPVITITGRRASPDAALFIGTGKAQEVAD
AVADLQLELVIFNHALSPAQQRNLERLLKVRVLDRTSLILDIFAQRAKSHEGKVQVELAQ
LQHLSTRLIRGWTHLERQKGGIGLRGPGETQLETDRRLLGERVKALRAVLAKLRRQHATQ
RRARGRNETFSVSLVGYTNAGKSTIFNALAKAGVYAANQLFATLDTTSRRVYLGEVGHVV
ISDTVGFIRELPHQLVEAFRATLEETIHADLLLHVVDAASPVRMEQIEEVNLVLKEIGAD
HVPQILVWNKIDAAGLEPTVEYDEYGKIQRVFVSAKSGAGLDLLREAIAASLKAALEARG
RSRSEPVDSVDMHA