Protein Info for HSERO_RS14535 in Herbaspirillum seropedicae SmR1

Annotation: nitrite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 857 PF07992: Pyr_redox_2" amino acids 1 to 302 (302 residues), 177.7 bits, see alignment E=1.1e-55 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 3 to 808 (806 residues), 1118.4 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 152 to 220 (69 residues), 54.8 bits, see alignment E=3.3e-18 PF18267: Rubredoxin_C" amino acids 328 to 398 (71 residues), 47.8 bits, see alignment E=3.4e-16 PF04324: Fer2_BFD" amino acids 432 to 479 (48 residues), 38.6 bits, see alignment 3.2e-13 PF03460: NIR_SIR_ferr" amino acids 568 to 630 (63 residues), 46.1 bits, see alignment 9.7e-16 PF01077: NIR_SIR" amino acids 640 to 764 (125 residues), 69.7 bits, see alignment E=6.2e-23

Best Hits

Swiss-Prot: 62% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 81% identity to bac:BamMC406_4400)

MetaCyc: 62% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IZP9 at UniProt or InterPro

Protein Sequence (857 amino acids)

>HSERO_RS14535 nitrite reductase (Herbaspirillum seropedicae SmR1)
MKIIVIGHGMVGHKFLECLAESGAGDLQVTILCEEPRPAYDRVHLSEFFAGKTAEDLSLV
APGFFDRGRSAVQFDLKLNAKAQQIDLERKQVLVNVAGVEETMSYDKLVLATGSYPFVPT
VAGNQRKDCFVYRTIEDLEAMLECGSRSKIGVVIGGGLLGLECAKALRDLKLETHVVEFA
PRLMAVQVDESGGRVLRQKIEELGVTAHTQKNTVEIVDGKHHTHRMNFEDGSHLETDMIV
FSAGIRPRDELARQAGLKVGDRGGIAIDNSCLTSDPAVYAIGECALWNGKIFGLVAPGYD
MARTAAKHLLSHRDEAIEVPAFNGADMSTKLKLMGVDVASIGDAHGKEAGSRSYQFTDER
KQIYKKIVVSESGKHLLGAVMVGDASEYGTLLQMMLNKIELPESPEFLILPQSDGKAKPG
LGVDALPESAQICSCNNVSKGQLCAAVASGVTNIGELKACTKAGTACGGCVPLVTQVMKA
EMKKQGLAVNNHVCEHFPYSRQEIHHLVRVGQIKSFDELLSKHGKGLGCDVCKPMVANVL
ASLWNDFVLKKEHAGLQDSNDYFLGNIQKDGTYSVVPRMAGGEVTPDGLIAVGQIAKKYG
LYTKITGGQRVDLFGARVDQLPDIWEELIAAGFETGHAYGKSLRTVKSCVGSTWCRYGVG
DSVGFAVRLENRYKGLRSPHKIKFGVSGCTRECAEAQGKDVGIIATEKGWNLYVCGNGGM
KPRHAELIASDLDEATLTRYVDRFLMFYVRTADRLQRTSTWRENLEGGLDYLKSVVLDDK
LGIAAELEAEMQRVVDTYECEWKKAVTDPETRKRFRHFVNSKAADGNIKFVEERGQIRPA
TVAEKKLLDIPVVVETV