Protein Info for HSERO_RS12750 in Herbaspirillum seropedicae SmR1
Annotation: acyl-CoA dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to ACAD8_MOUSE: Isobutyryl-CoA dehydrogenase, mitochondrial (Acad8) from Mus musculus
KEGG orthology group: None (inferred from 100% identity to hse:Hsero_2550)MetaCyc: 44% identical to short-chain acyl-CoA dehydrogenase monomer (Pseudomonas putida KT2440)
RXN-13449 [EC: 1.3.8.1]
Predicted SEED Role
"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)
MetaCyc Pathways
- oleate β-oxidation (31/35 steps found)
- superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
- β-alanine biosynthesis II (4/6 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
- valproate β-oxidation (5/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.2
Use Curated BLAST to search for 1.3.8.1 or 1.3.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See D8IWM9 at UniProt or InterPro
Protein Sequence (386 amino acids)
>HSERO_RS12750 acyl-CoA dehydrogenase (Herbaspirillum seropedicae SmR1) MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG GLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIG SYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVS AIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGG RINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA ATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQI LEGTNEIMRVIISRKLLDRDNLDDIR