Protein Info for HSERO_RS12510 in Herbaspirillum seropedicae SmR1

Annotation: glycogen branching protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 168 to 801 (634 residues), 903.6 bits, see alignment E=3.3e-276 PF02922: CBM_48" amino acids 184 to 267 (84 residues), 79.3 bits, see alignment E=3.2e-26 PF00128: Alpha-amylase" amino acids 338 to 412 (75 residues), 28.9 bits, see alignment E=1.4e-10 PF02806: Alpha-amylase_C" amino acids 700 to 802 (103 residues), 75.1 bits, see alignment E=7e-25

Best Hits

Swiss-Prot: 66% identical to GLGB_RALSO: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to hse:Hsero_2503)

MetaCyc: 52% identical to 1,4-alpha-glucan branching enzyme (Mycobacterium tuberculosis H37Rv)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IWI2 at UniProt or InterPro

Protein Sequence (804 amino acids)

>HSERO_RS12510 glycogen branching protein (Herbaspirillum seropedicae SmR1)
MSGMSVMAYRASVESPEASSDASFAPPSSASSADSIHPLETTALSLSFEEQGMLEALLAG
RLTQPFDLLGPRYASAKGDGAAVRCFHPGAEAVRVRCRHSGVLLAELQQYPVDGQPSGFF
SGLVPGLEQRRHEVPYVLEVDWRIPDGSRSVQSTEDPYAFGLLLGELDLHLLQEGRHRQL
ADCLGARPMEIDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPR
IGPGDVYKYEIRGRGGVLLPLKADPVARATEPPPATASVVADARPYVWQDQQWMAERAQR
QSLSAPMSIYEVHPASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELMPVMEHPFG
GSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTA
LYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDY
SRKEGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAEESTSWPGVTAPVKEEGD
HHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPISHDEVVHG
KRALLNKMPGDQWRRFANVRAYFGFMWTHPGKKLLFMGSEFGQPDEFDHDKSPYWHLLDE
HAGHPAEHRQLRQLVGDLNRLYCSEPALHQRDCRADGFAWAVGDDSVNSVLAYFRYADEG
PPVLVVVNLTPVPRVNYRIGVPSIAPGHHGVWREVLNTDAGLYGGSNNGNLGAVQVQELA
WHGHAASIELTLPPLSTLILRRES