Protein Info for HSERO_RS12315 in Herbaspirillum seropedicae SmR1
Annotation: cytochrome O ubiquinol oxidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 68% identical to CYOB_ECOLI: Cytochrome bo(3) ubiquinol oxidase subunit 1 (cyoB) from Escherichia coli (strain K12)
KEGG orthology group: K02298, cytochrome o ubiquinol oxidase subunit I [EC: 1.10.3.-] (inferred from 100% identity to hse:Hsero_2463)MetaCyc: 68% identical to cytochrome bo3 subunit 1 (Escherichia coli K-12 substr. MG1655)
RXN-21817 [EC: 7.1.1.3]
Predicted SEED Role
"Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-)" in subsystem Terminal cytochrome O ubiquinol oxidase or Terminal cytochrome oxidases (EC 1.10.3.-)
MetaCyc Pathways
- D-lactate to cytochrome bo oxidase electron transfer (2/2 steps found)
- NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
- glycerol-3-phosphate to cytochrome bo oxidase electron transfer (2/2 steps found)
- proline to cytochrome bo oxidase electron transfer (2/2 steps found)
- pyruvate to cytochrome bo oxidase electron transfer (2/2 steps found)
- succinate to cytochrome bo oxidase electron transfer (2/2 steps found)
- NADH to cytochrome bo oxidase electron transfer II (1/2 steps found)
Isozymes
Compare fitness of predicted isozymes for: 1.10.3.-
Use Curated BLAST to search for 1.10.3.- or 7.1.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See D8IWE2 at UniProt or InterPro
Protein Sequence (667 amino acids)
>HSERO_RS12315 cytochrome O ubiquinol oxidase (Herbaspirillum seropedicae SmR1) MSDNFDLIKLIFGRLSLEAIPYHEPILIGTFAMVALGGIVVLGLLTKYRLWGMLWRDWFT SIDHKRIGIMYIVLAIVMLLRGFADAVMMRAQQAIAFGDNAGFLPPHHYDQVFTAHGVIM IFFVAMPLVTGLMNYVVPLQIGARDVAFPFLNNFSFWMTTFGALLVMASLFVGEFARTGW LAYPPLSGILYSPDVGVDYYIWALQVAGVGTLLSGINLVVTIVKMRAPGMNMMKMPVFTW TSLCTNVLIVAAFPVLTAVLAMLSLDRVFGTNFFTNDMGGNAMMYVNLIWIWGHPEVYIL VLPAFGIFSEIVSTFSGKRLFGYTSMVYATVVITILSYLVWLHHFFTMGSGASVNSFFGI TTMIISIPTGAKIFNWLFTMYRGRIRFEVPMLWTIGFMVTFVIGGMTGVLLAVPPADFVL HNSLFLIAHFHNVIIGGVVFGMFAGINFWFPKAFGFKLNEFWGKCSFWFWLVGFYLAFMP LYVLGLMGVTRRMSHFDDPSLQIWFVVAAIGAGLIALGIGSFIIQIAVSIRDRKKLADVT GDPWNGRTLEWSTSSPPPAYNFAFTPVVHDNDAWADMKKNKYQRPLGDFVEIHMPKNTGA GFIIAALSAVLGFTLIWHMWLPAVATFVVLVAATIIHTFNYNRDYYIPADEVVRVEAERT RLLNSHV