Protein Info for HSERO_RS11650 in Herbaspirillum seropedicae SmR1

Annotation: glycogen branching protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 175 to 802 (628 residues), 900.7 bits, see alignment E=2.5e-275 PF02922: CBM_48" amino acids 192 to 274 (83 residues), 79.3 bits, see alignment E=3.3e-26 PF00128: Alpha-amylase" amino acids 340 to 417 (78 residues), 24.2 bits, see alignment E=3.7e-09 PF02806: Alpha-amylase_C" amino acids 702 to 803 (102 residues), 81.3 bits, see alignment E=8e-27

Best Hits

Swiss-Prot: 66% identical to GLGB_RALSO: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_2327)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IV87 at UniProt or InterPro

Protein Sequence (804 amino acids)

>HSERO_RS11650 glycogen branching protein (Herbaspirillum seropedicae SmR1)
MPASRRKSLTEAPGAPPSEVVLDESLVARLMQGRLGDPFALLGPREIADAPEATQKAKAK
RLRLCCYFPGASAVQVYARTSAAPDELHAPDSTGSLLGSLHEWSPATGQGESDHDQEAPR
QRSGVFFGEVKLPRRAVQPPIPYVFHVTWRGADGRETLQITEDAYAFGLLLSDFDLHLFR
EGRHRQLARCLGSQVMQIDGVEGVRFAVWAPNAQRVSVIGDFNQWDGRRNPMRLRQSAGV
WELFVPRLGDGALYKYEIIGPHGELLPGKADPMARATEVPPATASRVIDDSKLVSGRRWQ
DAHWLQQRAQGQTRQAPMSIYEVHAASWLRLPGEGNRNLDWNELADRLIPYVKGMGFTHV
ELLPVMEHPFGGSWGYQPLSQFAPSARFGPPAEFATFVDACHRAGIGVLLDWVPAHFPSD
PHGLAYFDGTPLYEHGDPREGIHQDWNTHIYNLGRHEVRGFLIASALEWLEHFHVDGLRV
DAVASMLYRDYSRSSGQWVPNIHGGRENLEAVDFLRELNQTVAERCPGALMIAEESTAWP
GVTAPVSEGGLGFTYKWNMGWMHDTLRYVELDSIYRRYHHHDITFGMIYAFSESFVLPIS
HDEVVHGKGTLLQKMAGDAWQRFANLRAYLGFMWTHPGKKLLFMGCEFAETREFDPDRSP
HWDLLDDAHHRGVQRLVRDLNRLYAGTPALYRHDTEAKGFSWVVGNDELNSVFAYLRQTD
DEAPVLVVINMTPVPRHHYRIGVPPGQTQQDRWEEILNTDADCYGGSHLGNGGQLRAQAQ
ACHGHASSLVLTLPPLSTLVLRRG