Protein Info for HSERO_RS11645 in Herbaspirillum seropedicae SmR1

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1121 transmembrane" amino acids 535 to 551 (17 residues), see Phobius details amino acids 692 to 712 (21 residues), see Phobius details TIGR02456: trehalose synthase" amino acids 9 to 551 (543 residues), 923.1 bits, see alignment E=5e-282 PF00128: Alpha-amylase" amino acids 32 to 385 (354 residues), 250 bits, see alignment E=7.2e-78 PF16657: Malt_amylase_C" amino acids 464 to 548 (85 residues), 89.7 bits, see alignment 1.6e-29 TIGR02457: putative maltokinase" amino acids 568 to 1108 (541 residues), 526.4 bits, see alignment E=8.4e-162 PF18085: Mak_N_cap" amino acids 600 to 672 (73 residues), 26.7 bits, see alignment 1.5e-09

Best Hits

Predicted SEED Role

"Trehalose synthase (EC 5.4.99.16)" in subsystem Trehalose Biosynthesis (EC 5.4.99.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IV86 at UniProt or InterPro

Protein Sequence (1121 amino acids)

>HSERO_RS11645 alpha-amylase (Herbaspirillum seropedicae SmR1)
MQFTRDPLWYKDAVIYQIHVKSYFDANDDGIGDFAGLIQKLDYITGLGVNTIWLLPFYPS
PRRDDGYDISEYKNVHPDYGNMSDVRRFIAAAHEHGLRVITELVINHTSDQHPWFQRARR
ARPGSVARNFYVWSDDDKSYADTRIIFVDTEKSNWTWDPVAKAYFWHRFYSHQPDLNFDN
PHVLKAVLNVMSFWLDLGIDGLRLDAVPYLIEREGTSNENLPETHAILKRIRAEMDSKYP
DRMLLAEANMWPEDVQQYFGDSDECHMAFHFPLMPRMYMALASQDRFPITDILRQTPDIP
ADCQWAIFLRNHDELTLEMVTDAERDYLWNHYAPDRRARINLGIRRRLAPLVERDWRRIQ
LLNSFLLSMPGTPVIYYGDEIGMGDNIHLGDRDGVRTPMQWTPDRNGGFSRVDPARLVLP
LLMDPQYGYQTINVEAQSADRHSMLNWMRRLLNVRKQHQAFGRGSLALVYPSNRKIFAYL
REFTDPRPGGATETILCVANVSQSAQAVELDLSAFAGRIPIEMMGGAAFPPIGQLNYLLT
LPPFGFYWFLLAPEANMPSWRAPTPEPLPEYVTLVLRKGVQEIMEPPARKLLEQEVLPHY
LLKRRWYAGKQRQIDKVEMREATTLPMSRTRPGALPIVLTEIDVHSSGTEGDAGAGSVQR
YLLPLGFIREEDTVSALPHQLALSRVRRGRNVGFLTDAFALDSFIVAVLELLADSASIKT
RSGQLRCIPTAAFDPAILTTALELRRITQEQSNSSVVINNVMMMKVLRQVNPGIHPEIEM
GRYLTERGFANVAATLGEVVRVADDGTPYALIVLQRYVDNQGDAWQWSMDTLERAIQSEA
LDESQRQPNTRPQDAQGIDDNSALVELVTFSRLLGQRLGEMHSLLGQPSDDPAFDPQTIG
EPVWRSWAQGAAQQLEAALDILRQKEEWPDAAEAHHAARLLAQRERLLERIHPLAQAAEG
SLAMRLHGDLHLGQVLIAFNDVYIVDFEGEPARPLEQRRAKGSPLRDVAGLLRSFDYAAA
FGHTMGPTDLDEAALASKKQILQRFAPACQAALLEGYRLGNPLLEQSLPLDAQALLLDLF
TLEKAAYEICYEAANRPTWMHVPLRGLYLIANRILGEEDGA