Protein Info for HSERO_RS10625 in Herbaspirillum seropedicae SmR1

Annotation: FMN-dependent NADH-azoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 PF02525: Flavodoxin_2" amino acids 3 to 200 (198 residues), 188.8 bits, see alignment E=9.5e-60 PF03358: FMN_red" amino acids 7 to 131 (125 residues), 37.3 bits, see alignment E=2e-13

Best Hits

Swiss-Prot: 55% identical to AZOR_HERAR: FMN-dependent NADH-azoreductase (azoR) from Herminiimonas arsenicoxydans

KEGG orthology group: K01118, FMN-dependent NADH-azoreductase [EC: 1.7.-.-] (inferred from 100% identity to hse:Hsero_2126)

MetaCyc: 48% identical to FMN dependent NADH:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
RXN0-5375 [EC: 1.7.1.17]; 1.6.5.- [EC: 1.7.1.17]

Predicted SEED Role

"FMN-dependent NADH-azoreductase"

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.- or 1.7.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IT67 at UniProt or InterPro

Protein Sequence (202 amino acids)

>HSERO_RS10625 FMN-dependent NADH-azoreductase (Herbaspirillum seropedicae SmR1)
MSTLLHIDSSARSGGSISRQLTASFVSQWQAKNPGGKVVHRDLAANALPHLSESMLGAYF
TPADARSAEQAEVIKQSDALVDELLAADTIVIGVPMYNFAPPSTLKAWIDHVFRAGRTFK
YTETGPVGLVTGKKAVIILSRGGMYSEGPMEALDFQGKYLKSALGFIGITDVELVVAEGV
SMGEEAAKQAVASAEAKINASV