Protein Info for HSERO_RS10245 in Herbaspirillum seropedicae SmR1

Annotation: hemolysin secretion protein D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 41 to 270 (230 residues), 116.1 bits, see alignment E=8.2e-38 PF16576: HlyD_D23" amino acids 52 to 267 (216 residues), 52.9 bits, see alignment E=6.4e-18 PF13533: Biotin_lipoyl_2" amino acids 63 to 107 (45 residues), 31.9 bits, see alignment 1.8e-11 PF13437: HlyD_3" amino acids 188 to 275 (88 residues), 42.5 bits, see alignment E=1.9e-14

Best Hits

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 100% identity to hse:Hsero_2046)

Predicted SEED Role

"Macrolide-specific efflux protein MacA" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISY7 at UniProt or InterPro

Protein Sequence (405 amino acids)

>HSERO_RS10245 hemolysin secretion protein D (Herbaspirillum seropedicae SmR1)
MKLAQLRRPKFIFLALLVLLIAAWIIRSVLTPPAPPTYLSATARVADIQDVVLASGTVKA
YKQVSVGAQVSGQIKSLKVALGDQVKKGQLVAEIDSLTQANALASAEFSLQNLQAQLRAK
EASLKQAQLAYARQKMMLAGDASSRENFESAEATLNTTQADIAALQAQIKDGAIKVDTAR
LNLGYTRISSPIEGQVVAIVAQEGQTVNANQSTPTIIKVARMDTVTIKAQISEADVVRVK
PGQPVFFTILGDPDHRYRTTLRAIEPAPDSILQDDTSSSTTSITSSSSASSTAIYYNGLL
DVPNPDGKLRISMTTQVNIVLSEASNALVLPSTALGAKAADGSYTVRVLDDQGQAHERKV
RIGINTNALVQIVEGVKAGERVVTGTVLPGAAASSSAHDGPPPHM