Protein Info for HSERO_RS09905 in Herbaspirillum seropedicae SmR1

Annotation: phenylalanyl-tRNA synthetase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 808 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 807 (807 residues), 775.7 bits, see alignment E=2.8e-237 PF01588: tRNA_bind" amino acids 45 to 150 (106 residues), 81.1 bits, see alignment E=1.3e-26 PF03483: B3_4" amino acids 218 to 390 (173 residues), 215.3 bits, see alignment E=1.2e-67 PF03484: B5" amino acids 412 to 477 (66 residues), 75.8 bits, see alignment E=6.4e-25 PF17759: tRNA_synthFbeta" amino acids 480 to 693 (214 residues), 201.4 bits, see alignment E=3.2e-63 PF03147: FDX-ACB" amino acids 709 to 807 (99 residues), 84.7 bits, see alignment E=1.2e-27

Best Hits

Swiss-Prot: 67% identical to SYFB_CUPNJ: Phenylalanine--tRNA ligase beta subunit (pheT) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 100% identity to hse:Hsero_1982)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISD9 at UniProt or InterPro

Protein Sequence (808 amino acids)

>HSERO_RS09905 phenylalanyl-tRNA synthetase subunit beta (Herbaspirillum seropedicae SmR1)
MQFSENWLRTMVNPTMTSDELSHLLTMSGLEVEEVEPVAPPFTNVVVGEVLEVAKHPDAD
RLNVCRVNVHTGTILNIVCGAPNVRAGLKVPCAMVGAVLPPGPDGKPFEIKVGKLRGVES
QGMLCSARELKLSEDHGGLLELPDDAPVGQNFRDYLQLNDLKFTIKLTPNKADCLSVLGV
AREVSALTGTPLKAPTFQSAAVNLDEKLPVRISAPDLCGRFAGRVIRGLNAKAATPQWMK
QRLERSGQRPISALVDISNYVMLELGRPSHVFDLDKIHGSLDVRWGRKGESLKLLNGNTV
EVDEWVGVISDDKELESLAGIMGGDATAVSLDTTNIYLEAAFWFPQAIQGRARRYNFSTD
AAHRFERGVDFATVVDHIERITALLLEICGTAETRVGPVDDQIVNLPKRAPVQLRTARAA
KVIGVEITDEMVSDIFKRLGLSFDYQPGLFSVTPPSYRFDIEIEEDLIEEVARVYGFENI
PALPPVAAHAMRIAPENTRSLFAIRRLLADQDYQEVVNFSFVESAWEADFAGNTDPIKLL
NPIASQMSVMRSSLIAGLVANARYNVNRKAARIRVFEVGAVFSKNAAVADGPLAVAGFDQ
PKRVAALAYGPVAEEQWGQASRNVDYFDVKADLEALFAPRTLRFVKSEHPALHPGRTAQV
LLGEQVIGVIGELHPKWQQKYDLPLAPVVFEVDADALQQRDLPTYAEISKFPAVSRDIAL
VVKQSVGAQQLMDVFAAEKQANPACQILQALVLFDEYQGRGLESDEKSLAFRCTLQDTQN
TLQDDKVDLAMNALVAAASAKLGARLRA