Protein Info for HSERO_RS08385 in Herbaspirillum seropedicae SmR1

Annotation: anaerobic (selenocysteine-containing) formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 14 to 767 (754 residues), 967.4 bits, E=2.4e-295
• PF00384: Molybdopterin
  • amino acids 111 to 494 (384 residues), 72.2 bits, E=4.1e-24
• PF01568: Molydop_binding
  • amino acids 654 to 759 (106 residues), 43 bits, E=4.2e-15

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to hse:Hsero_1686)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• oxalate degradation VI (1/4 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

See D8IQT8 at UniProt or InterPro

Protein Sequence (775 amino acids)

>HSERO_RS08385 anaerobic (selenocysteine-containing) formate dehydrogenase (Herbaspirillum seropedicae SmR1)
MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGRGLRTLLSQNQPDGFDCPGCAWPDR
EHTSTFEFCENGVKAVAAEATKKRVTPEFFAQHTVTELMQQSDFELEEHGRLTHPMVYDA
ATDKYKKIEWADAFALMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD
CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG
ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIEL
DDAARAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIVAESGVSREDIEKLAQVYVK
GKAVIATWGMGLTQHKHAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID
EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF
EAMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDMQKSGPQGVTVEDSMSMVHVS
YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEQVYDAFKG
YNERIANPGGFHLGVASRDRVWKTASGKANFIVHDIPKDTPIHRARAIHGEKLLTLMTTR
SHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSVWDDGVERRAEG
FLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSRSAVQRSAA