Protein Info for HSERO_RS06720 in Herbaspirillum seropedicae SmR1

Annotation: Rieske (2Fe-2S) protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 47 to 130 (84 residues), 77.7 bits, E=5.1e-26
• PF00848: Ring_hydroxyl_A
  • amino acids 192 to 427 (236 residues), 60.4 bits, E=2.4e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_1341)

MetaCyc: 64% identical to 2-(3-hydroxy-2-oxo-1H-indol-3-yl)acetate dioxygenase IacC subunit (Pseudomonas putida 1290)
RXN-20920

Predicted SEED Role

"Ring hydroxylating dioxygenase, alpha subunit/Rieske (2Fe-2S) protein (EC 1.14.12.18)" (EC 1.14.12.18)

MetaCyc Pathways

• indole-3-acetate degradation I (3/3 steps found)
• diphenyl ethers degradation (3/4 steps found)
• biphenyl degradation (3/5 steps found)

KEGG Metabolic Maps

• Biphenyl degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.12.18

Sequence Analysis Tools

See D8IP37 at UniProt or InterPro

Protein Sequence (428 amino acids)

>HSERO_RS06720 Rieske (2Fe-2S) protein (Herbaspirillum seropedicae SmR1)
MNAPLRFHPRKTDSSYADLVQDAVVDPSLYTDPALFEAEMDKIFYKTWIWVAHESEIKNP
GDFKTAQIGRQPVIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLD
GKLRGLPYPEGYEDVIEKADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLF
MKQGAGFPIKVQGEHKFSFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTD
DQSITRSLGNGHSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSL
HGAGFNLNLFPNVAMSMSFFRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQG
PFGFGSPDDAEAWERVQAGSHAGRDAPILVNRGLNREWVDDNGDKVSHSTDETGMREAYA
MWKKMMEK