Protein Info for HSERO_RS06175 in Herbaspirillum seropedicae SmR1

Annotation: queuine tRNA-ribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 TIGR00430: tRNA-guanine transglycosylase" amino acids 4 to 369 (366 residues), 551 bits, see alignment E=1.2e-169 TIGR00449: tRNA-guanine family transglycosylase" amino acids 4 to 370 (367 residues), 526.1 bits, see alignment E=4.2e-162 PF01702: TGT" amino acids 12 to 371 (360 residues), 553.8 bits, see alignment E=8.4e-171

Best Hits

Swiss-Prot: 84% identical to TGT_JANMA: Queuine tRNA-ribosyltransferase (tgt) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 100% identity to hse:Hsero_1229)

MetaCyc: 64% identical to tRNA-guanine transglycosylase (Escherichia coli K-12 substr. MG1655)
tRNA-guanine transglycosylase. [EC: 2.4.2.29]

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8INS5 at UniProt or InterPro

Protein Sequence (376 amino acids)

>HSERO_RS06175 queuine tRNA-ribosyltransferase (Herbaspirillum seropedicae SmR1)
MLEFTLLKTDGKARRGRVKLNHGTVETPIFMPVGTYGSVKAMSPLELKEINAQIILGNTF
HLWLRPGLEVVSRFGGLHKFIGWDKPILTDSGGFQVFSLGEMRKITEEGVHFASPINGDR
LFLSPEISMQIQRVLNSDIVMQFDECTPYEIDGRPATREEAGKSMRMSLRWAKRSKDEFD
RGENPNALFGIVQGGMFEGLRDESLAGLEELGFDGFAIGGLSVGEPKEDMMRVLEHVGPR
LPADKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWIFTRYGDVKIKNAKYKND
DRPFDESCDCYACKNFSRAYLHHLHRAGEILGARMNTVHNLHYYLQLMQEMRDAIDAGTF
QDFVKKFKEDRARGVD