Protein Info for HSERO_RS05420 in Herbaspirillum seropedicae SmR1

Annotation: 4-aminobutyrate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 TIGR00700: 4-aminobutyrate transaminase" amino acids 12 to 423 (412 residues), 640.4 bits, see alignment E=5.2e-197 PF00202: Aminotran_3" amino acids 25 to 423 (399 residues), 440 bits, see alignment E=3.7e-136

Best Hits

Swiss-Prot: 69% identical to PUUE_ECOLI: 4-aminobutyrate aminotransferase PuuE (puuE) from Escherichia coli (strain K12)

KEGG orthology group: K00823, 4-aminobutyrate aminotransferase [EC: 2.6.1.19] (inferred from 100% identity to hse:Hsero_1082)

MetaCyc: 72% identical to 4-aminobutyrate aminotransferase monomer (Cupriavidus necator H16)
4-aminobutyrate transaminase. [EC: 2.6.1.19]; 2.6.1.- [EC: 2.6.1.19]

Predicted SEED Role

"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.19

Use Curated BLAST to search for 2.6.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8J1H8 at UniProt or InterPro

Protein Sequence (426 amino acids)

>HSERO_RS05420 4-aminobutyrate aminotransferase (Herbaspirillum seropedicae SmR1)
MTSKANNQELQQRKNAATPRGVGVMCDFYAERAANAELWDVEGRRFIDFAAGIAVLNTGH
RHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAERINRLTPGNYPKKTAFFSTGAEAVEN
AIKIARAHTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSALHG
ITSEDALEAVKGLFKSDIEAKRVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLI
ADEVQSGYGRTGKLFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIMDAPAPGGLGGT
YAGNPLAIASALAVLDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAV
EFADPATGKPDAEYTKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDEALGILA
KAIRLA