Protein Info for HSERO_RS05150 in Herbaspirillum seropedicae SmR1

Annotation: galactonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02746: MR_MLE_N
  • amino acids 15 to 107 (93 residues), 91.9 bits, E=3.3e-30
• PF13378: MR_MLE_C
  • amino acids 128 to 355 (228 residues), 242.9 bits, E=3.1e-76

Best Hits

Swiss-Prot: 63% identical to DGOD_RALPJ: D-galactonate dehydratase (dgoD) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01684, galactonate dehydratase [EC: 4.2.1.6] (inferred from 100% identity to hse:Hsero_1029)

MetaCyc: 60% identical to D-galactonate dehydratase (Escherichia coli K-12 substr. MG1655)
Galactonate dehydratase. [EC: 4.2.1.140, 4.2.1.6]

Predicted SEED Role

"Gluconate dehydratase (EC 4.2.1.39)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.39)

MetaCyc Pathways

• D-galactonate degradation (3/3 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.140 or 4.2.1.39 or 4.2.1.6

Sequence Analysis Tools

See D8J0Z1 at UniProt or InterPro

Protein Sequence (382 amino acids)

>HSERO_RS05150 galactonate dehydratase (Herbaspirillum seropedicae SmR1)
MKITKITTFIVPPRWCFLKIETDEGVVGWGEPIVEGRAHSVAAAVEELSDYLIGKDPRNI
EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSWI
GGDRPADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI
GVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSR
WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD
AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR
AEVGHRWRNPIWRHKDGSFAEW