Protein Info for HSERO_RS03520 in Herbaspirillum seropedicae SmR1

Annotation: aminoglycoside phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 PF01636: APH" amino acids 31 to 259 (229 residues), 144.5 bits, see alignment E=2.5e-46

Best Hits

Swiss-Prot: 54% identical to AMGK_NEIMB: N-acetylmuramate/N-acetylglucosamine kinase (amgK) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K07102, (no description) (inferred from 100% identity to hse:Hsero_0704)

MetaCyc: 50% identical to N-acetyl-D-muramate 1-kinase (Pseudomonas putida KT2440)
RXN-18660 [EC: 2.7.1.221]; N-acetylhexosamine 1-kinase. [EC: 2.7.1.221, 2.7.1.162]; N-acetylgalactosamine kinase. [EC: 2.7.1.221, 2.7.1.162, 2.7.1.157]

Predicted SEED Role

"COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases" in subsystem YjeE

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.157 or 2.7.1.162 or 2.7.1.221

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IZA3 at UniProt or InterPro

Protein Sequence (343 amino acids)

>HSERO_RS03520 aminoglycoside phosphotransferase (Herbaspirillum seropedicae SmR1)
MSSPGSPPDPRLSELHTWLASLADTPVLPATLRPASADASFRRYFRVDTPDGGSAIVMDA
PPPQEDVRPFVEVAGLFAATGVTVPKVLAQDCERGFLLLSDLGSTTYLSQLSADSAHALY
MDAIEALVLLQAQSKPEALPEYDRPLLLRELELFREWYIGKHLGVTLTDEQNAVLDKTFD
AILANNLAQPQVYVHRDYHSRNLMVLPKGNPGILDFQDAVYGPITYDLVSLLRDAYIQWD
EEMVLDWAIRYWEKARRAGLPVTPDIDTFYRDFEWMGLQRHLKVLGIFARLWHRDGKDGY
LKDIPLVMEYTLKTARRYVAFTPLVRLIEKLEQEHAPKFGYTF