Protein Info for HSERO_RS03215 in Herbaspirillum seropedicae SmR1

Annotation: sodium:solute symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 43 to 61 (19 residues), see Phobius details amino acids 82 to 104 (23 residues), see Phobius details amino acids 110 to 131 (22 residues), see Phobius details amino acids 158 to 182 (25 residues), see Phobius details amino acids 192 to 213 (22 residues), see Phobius details amino acids 222 to 241 (20 residues), see Phobius details amino acids 380 to 402 (23 residues), see Phobius details amino acids 414 to 435 (22 residues), see Phobius details amino acids 503 to 530 (28 residues), see Phobius details amino acids 552 to 570 (19 residues), see Phobius details amino acids 576 to 600 (25 residues), see Phobius details amino acids 607 to 625 (19 residues), see Phobius details amino acids 641 to 665 (25 residues), see Phobius details TIGR03648: probable sodium:solute symporter, VC_2705 subfamily" amino acids 44 to 683 (640 residues), 735.9 bits, see alignment E=1.3e-225 PF00474: SSF" amino acids 69 to 236 (168 residues), 100.9 bits, see alignment E=3.9e-33 amino acids 501 to 616 (116 residues), 72.9 bits, see alignment E=1.3e-24

Best Hits

Swiss-Prot: 53% identical to Y2524_CUPNH: Uncharacterized symporter H16_A2524 (H16_A2524) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K14393, cation/acetate symporter (inferred from 100% identity to hse:Hsero_0643)

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IYK2 at UniProt or InterPro

Protein Sequence (683 amino acids)

>HSERO_RS03215 sodium:solute symporter (Herbaspirillum seropedicae SmR1)
MESNKDFFRRLCRYYGFYTLGFIAFLCVLAILEREGMPRTWIGYLFLFVTIGLYAGIGVV
CRTSDVPEYYVAGRRVPPLFNGMATAADWISAATFISLAGGLYLQGFDGLAYILGWTGGY
CLVALLIAPYLRKFGQYTIADFLAARYPGRPGSQGVNVIRILAVAATILISFTYVVAQIY
GVGLITSRFTGIDFSIGIFLGLASILVCSFLGGMRAITWTQVAQYIIILLAYLIPVIWLS
AKHTTVPVPPVAYGAVLPKLSAIEQRLEDDPKEKEIRKIFQARADDYERKLRNLPQSWVD
GRLEAHTNADQVRRDNHASLAEIKAASRALSSYPKSPEEAERKWTEARAANLARAQPPVS
QTEPFPGRDQESSDIKRNNFLALVFCLMLGTAALPHILMRYYTTPTVAGARTSVFWTLFF
IMLIYLTVPALTVLVKYDIYTSLVGSSYSHLPDWVYYWANMDKLNPLVSITDLNRDGLVQ
LGEISMDGDIIVLAMPEIAGLPYFVSGLVAAGGLAAALSTADGLLLTISNALSHDVYYKV
VDPSASTQKRVTISKLLLLVVALLAAYAASLKPGDILSMVGAAFSLAASTLFPALVLGVF
WKRANQAGAIAGILCGFLMCVYYMLHTNPSFGGSAAGQWFHIAPISAGIFGVPVGMAAMV
IVSLLTPPPDEATIALIDHIRTP