Protein Info for HSERO_RS01815 in Herbaspirillum seropedicae SmR1

Annotation: gamma-glutamyl kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 9 to 237 (229 residues), 164.7 bits, E=3e-52
• TIGR01027: glutamate 5-kinase
  • amino acids 9 to 370 (362 residues), 434.8 bits, E=1.4e-134
• PF01472: PUA
  • amino acids 281 to 355 (75 residues), 76 bits, E=1.7e-25

Best Hits

Swiss-Prot: 84% identical to PROB_HERAR: Glutamate 5-kinase (proB) from Herminiimonas arsenicoxydans

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 100% identity to hse:Hsero_0367)

MetaCyc: 46% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)
• L-ornithine biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Sequence Analysis Tools

See D8IW94 at UniProt or InterPro

Protein Sequence (372 amino acids)

>HSERO_RS01815 gamma-glutamyl kinase (Herbaspirillum seropedicae SmR1)
MQSVIQRAKRLIIKVGSSLVTNDGKGLDANAIARWAEQIAQLRALGKEVVLVSSGAVAEG
MQRLGFEKRPTGIHHLQACAAVGQMGLAQIYETSFRKHDLHTAQVLLTHADLADRERYLN
ARSTLFALLQLGVVPVINENDTVVTDEIKFGDNDTLGALVANLIEAEALIILTDQPGLFT
ADPRKDPTATLISEARAGDPSLELIAGGTGTGIGRGGMLTKILAAKRAATSGAHTVIAWG
REEQVLTRLAAGEAIGTQLNAETAQLTARKQWMADHLKTAGRVVLDAGAVQKLSLEGKSL
LPIGVVEVSGEFGRGDVITCVDENGRAIARGMSNYNSSDARRIIRHPSSEIQAILGFVEE
PELVHRDNLVLL