Protein Info for HSERO_RS01715 in Herbaspirillum seropedicae SmR1

Annotation: preprotein translocase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 918 PF07517: SecA_DEAD" amino acids 5 to 404 (400 residues), 438.9 bits, see alignment E=2.5e-135 TIGR00963: preprotein translocase, SecA subunit" amino acids 26 to 825 (800 residues), 1137.8 bits, see alignment E=0 PF00270: DEAD" amino acids 97 to 214 (118 residues), 26.2 bits, see alignment E=1.9e-09 PF01043: SecA_PP_bind" amino acids 230 to 360 (131 residues), 132.9 bits, see alignment E=2.2e-42 PF21090: P-loop_SecA" amino acids 419 to 624 (206 residues), 311.3 bits, see alignment E=9e-97 PF07516: SecA_SW" amino acids 626 to 838 (213 residues), 235.7 bits, see alignment E=1.6e-73 PF02810: SEC-C" amino acids 898 to 916 (19 residues), 40 bits, see alignment (E = 8.5e-14)

Best Hits

Swiss-Prot: 73% identical to SECA_BURA4: Protein translocase subunit SecA (secA) from Burkholderia ambifaria (strain MC40-6)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 73% identity to bac:BamMC406_0497)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IW74 at UniProt or InterPro

Protein Sequence (918 amino acids)

>HSERO_RS01715 preprotein translocase subunit SecA (Herbaspirillum seropedicae SmR1)
MSFLTKIFGSRNQRLLKQYQKIVRQINALEPTVEKLTDAELQAKTPEFKQRIAGGETLDA
ILPEAFAVCREASRRVLKMRHFDVQLIGGMALHFGKIAEMRTGEGKTLMATLPAYLNALA
GKGVHVVTVNDYLAQRDAEDMGRLYSWLGLTTGVNLSQMEHDVKQEAYAADITYGTNNEF
GFDYLRDNMVFDADDRVQRALNFAIVDEVDSILIDEARTPLIISGQAENHTELYHKMNAV
PPLLTQQIGEETPDGKGKVEVPGDYTKDEKSHQVLLTEAGHDKAEEILTQMGLLPEGASL
YDAANITLIHHLYAALRAHTLYHRDQHYVVQNGEVVIVDEFTGRLMTGRRWSDGLHQAVE
AKEGVKIQNENQTLASITFQNYFRMYAKLSGMTGTADTEAYEFQEIYHLETVVIPPNRPN
ARKDRQDQVYKTAQEKYMAMLKDIQDCYERGQPVLVGTTSIENSELLSGILTKAKLPHNV
LNAKQHAREAEIVAQAGRPKMITIATNMAGRGTDIVLGGNVGKQIQLIEADESLSDADKA
AKAQQLRDEWQSLHDHVVNAGGLHIIGTERHESRRVDNQLRGRSGRQGDPGSSRFYLSLD
DALLRIFAGDRVRAIMDRLKMPEGEPIEAGIVTRSIESAQRKVEARNFDIRKQLLEYDDV
ANDQRKVIYQQRNELLESAEMAEMIASLRDGVFTDLFREYVPAESMEEQWDIPGLQAVLK
NEWQLDVYLDATLKAEPDMADDELLAHLLKAAKDAYDAKVAVVGVEAFAGFERSVMLQSI
DNHWREHLAALDHLRQGIHLRGYAQKNPKQEYKREAFELFAQMLDLIKNEVIKVVMTVRI
QSREEIEAAEESMAGGSPINVNYQHADFDPEAAPEELLAPTVQAGSGDDGSEYAKVGRND
PCPCGSGKKYKQCHGKLA