Protein Info for HSERO_RS01260 in Herbaspirillum seropedicae SmR1

Annotation: 3-hydroxyacyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02737: 3HCDH_N
  • amino acids 8 to 188 (181 residues), 148.3 bits, E=4.6e-47
• PF00725: 3HCDH
  • amino acids 194 to 293 (100 residues), 53.8 bits, E=4.6e-18
• PF16113: ECH_2
  • amino acids 491 to 624 (134 residues), 47.3 bits, E=4.3e-16
• PF00378: ECH_1
  • amino acids 499 to 637 (139 residues), 50 bits, E=5.5e-17

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 100% identity to hse:Hsero_0255)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.3.3.8)

MetaCyc Pathways

• oleate β-oxidation (31/35 steps found)
• superpathway of phenylethylamine degradation (11/11 steps found)
• phenylacetate degradation I (aerobic) (9/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (5/5 steps found)
• adipate degradation (5/5 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• oleate β-oxidation (thioesterase-dependent, yeast) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• valproate β-oxidation (5/9 steps found)
• glycerol degradation to butanol (10/16 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (2/5 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (4/10 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• 2-methylpropene degradation (2/8 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• gallate degradation III (anaerobic) (3/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• L-tryptophan degradation III (eukaryotic) (3/15 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• superpathway of testosterone and androsterone degradation (7/28 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.3.3.8

Sequence Analysis Tools

See D8IVY3 at UniProt or InterPro

Protein Sequence (795 amino acids)

>HSERO_RS01260 3-hydroxyacyl-CoA dehydrogenase (Herbaspirillum seropedicae SmR1)
VSNFIVKKVAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSP
APLGNKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFAS
NTSGLSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTT
LGKGVVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTA
DVVGLDTMGHVIRTMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVGKDIL
RIDPAKGDYVPGGAKADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFHYIAV
HLESVADNARDLDFAMRWGFGWSVGPFETWQAAGWKQIAQWVKEDIDAGKALSTAPLPDW
VFDGRDGVHSEKGSYSPSKKADVARSELPVYQRQAFRAPLVGEGAADGKSAGTTFFEDES
VRIWHQNDDVLILSFKTKMHVIGQGVIDGMNKAVSEAEQNFKGLVIWHPDAAEGGAFSAG
ADLQSMLPLFMSGGVKAIEPVVHQLQQAHQRLKYASVPVIAAVAGLALGGGCELLLHTAK
RVVSIESYIGLVEVGVGLIPAGGGLKEAAVRAAREAKGNDILPFLKEYMLAAATANVSKS
ALEARKLGYLREDDVIVFNPYELLHVAKVEARALFDAGYRPQLPPQVPVIGRNGLATIMA
QLVNMRDGGFISAHDFKLGKMIAETVSGGEVEEGSIVNEQWLLDIERKFFMELLNHPKTQ
ERIMGMMQTGKPVRN